Barbaro K C, Cardoso J L, Eickstedt V R, Mota I
Centro de Pesquisa e Formação em Imunologia, Instituto Butantan, São Paulo, Brazil.
Toxicon. 1992 Mar;30(3):331-8. doi: 10.1016/0041-0101(92)90873-4.
Loxosceles gaucho spider venom causes a typical dermonecrotic lesion in bitten patients and rarely causes lethal systemic effects. Gel filtration on Sephadex G 100 of L. gaucho spider venom resulted in three fractions: fraction A, containing the higher mol. wt components (approximately 35,000); fraction B, containing lower mol. wt components (approximately 15,000); and fraction C, containing very low mol. wt components (probably small peptides). The dermonecrotic and lethal activities were detected exclusively in fraction A. The venom and fraction A produced large dermonecrotic lesions in rabbits with necrosis spreading by gravity to the skin of the lateral body wall. Analysis by SDS-PAGE showed that the proteins contained in fraction A are approximately 35,000 and 33,000 mol. wt. Immunoblotting analysis showed that the proteins responsible for the dermonecrotic and lethal activity are very immunogenic and the first to be detected by antibodies during the course of immunization.
高乔隐士蜘蛛毒液会在被叮咬的患者身上引发典型的皮肤坏死性损伤，且很少引起致命的全身效应。对高乔隐士蜘蛛毒液进行葡聚糖凝胶G 100柱层析，得到三个组分：组分A，含有较高分子量的成分（约35,000）；组分B，含有较低分子量的成分（约15,000）；以及组分C，含有极低分子量的成分（可能是小肽）。皮肤坏死和致死活性仅在组分A中检测到。毒液和组分A在兔子身上产生了大的皮肤坏死性损伤，坏死通过重力扩散到身体侧壁的皮肤。SDS-PAGE分析表明，组分A中所含蛋白质的分子量约为35,000和33,000。免疫印迹分析表明，负责皮肤坏死和致死活性的蛋白质具有很强的免疫原性，并且在免疫过程中最早被抗体检测到。
