紫外线诱导的白三烯B4、12-羟基二十碳四烯酸和5-羟基二十碳四烯酸的转化与降解

Transformation and degradation of leukotriene B4, 12-hydroxyeicosatetraenoic acid and 5-hydroxyeicosatetraenoic acid induced by ultraviolet radiation.

作者信息

Lehmann B, Kämpf A, Barth J

机构信息

Department of Dermatology, Carl Gustav Carus Medical Academy, Dresden, Germany.

出版信息

Photodermatol Photoimmunol Photomed. 1992 Feb;9(1):8-14.

PMID:1327059

Abstract

To reveal the underlying mechanisms in the therapeutic effectiveness of ultraviolet (UBV)- and psoralen plus UVA phototherapy, the photostability of leukotriene B4 (LTB4), 12-hydroxyeicosatetraenoic acid (12-HETE) and 5-HETE was investigated in the presence and absence of photosensitizers (8-methoxypsoralen and 5-methoxypsoralen). Arachidonic lipoxygenase products were irradiated with doses of narrow-band UVB (311 nm) ranging from 0.5-3.2 J/cm2, UVB and UVA (305-400 nm) ranging from 0.6-6.3 J/cm2 and UVA ranging from 5.0-60.0 J/cm2, respectively. High-performance liquid chromatography demonstrated a dose-dependent decrease of LTB4, 12-HETE, and 5-HETE. The photostability of 12-HETE was much higher than that of LTB4 and 5-HETE. Two products of transformation of LTB4 were identified as 5(S), 12(R)-dihydroxy-(6E, 8E, 10E, 14Z)-eicosatetraenoic acid [6-trans-LTB4] and 5(S), 12(R)-dihydroxy-(6E, 8Z, 10E, 14Z)-eicosatetraenoic acid [5(S), 12(R)-DiHETE]. There was no significant increase in photodegradation after addition of photosensitizing psoralens.

摘要

为揭示紫外线（UVB）及补骨脂素加UVA光疗疗效的潜在机制，我们研究了在存在和不存在光敏剂（8-甲氧基补骨脂素和5-甲氧基补骨脂素）的情况下白三烯B4（LTB4）、12-羟基二十碳四烯酸（12-HETE）和5-羟基二十碳四烯酸（5-HETE）的光稳定性。分别用0.5 - 3.2 J/cm²的窄带UVB（311 nm）、0.6 - 6.3 J/cm²的UVB和UVA（305 - 400 nm）以及5.0 - 60.0 J/cm²的UVA照射花生四烯酸脂氧合酶产物。高效液相色谱显示LTB4、12-HETE和5-HETE呈剂量依赖性减少。12-HETE的光稳定性远高于LTB4和5-HETE。LTB4的两种转化产物被鉴定为5(S),12(R)-二羟基-(6E,8E,10E,14Z)-二十碳四烯酸[6-反式-LTB4]和5(S),12(R)-二羟基-(6E,8Z,10E,14Z)-二十碳四烯酸[5(S),12(R)-二氢二十碳四烯酸]。添加光敏性补骨脂素后光降解没有显著增加。