Chun S Y, Popliker M, Reich R, Tsafriri A
Department of Hormone Research, Bernhard Zondek Hormone Research Laboratory, Weizmann Institute of Science, Rehovot, Israel.
Biol Reprod. 1992 Aug;47(2):245-53. doi: 10.1095/biolreprod47.2.245.
Proteinases and their inhibitors control follicular connective tissue remodeling associated with follicular rupture. We examined the regulation and cellular localization of plasminogen activator inhibitor type-1 (PAI-1) and tissue inhibitor of metalloproteinase type-1 (TIMP-1) mRNAs by in situ hybridization. [35S]UTP-labeled RNA probes were hybridized to ovarian sections of eCG-primed immature rats treated with hCG. Before hCG stimulation of ovulation, very low expression of PAI-1 mRNA was observed in theca cells. After hCG administration, expression of PAI-1 mRNA was increased in theca cells of most antral follicles, whereas expression in granulosa cells was limited to preovulatory follicles and only to areas where the basal membrane was dissociated. Before hCG treatment, low expression of TIMP-1 mRNA was observed in theca cells, but not in granulosa cells. After hCG treatment, TIMP-1 mRNA was greatly stimulated in theca cells irrespective of follicle size, while the expression in granulosa cells was limited to large antral follicles. The present study demonstrates cell-specific expression of PAI-1 and TIMP-1 mRNAs in the LH/hCG-stimulated ovary, thus confirming the localized control of preovulatory proteolysis by coexpression of both enzymes and their respective inhibitors.
蛋白酶及其抑制剂控制与卵泡破裂相关的卵泡结缔组织重塑。我们通过原位杂交研究了纤溶酶原激活物抑制剂1型（PAI-1）和金属蛋白酶组织抑制剂1型（TIMP-1）mRNA的调控及细胞定位。[35S]UTP标记的RNA探针与经hCG处理的eCG预处理未成熟大鼠的卵巢切片杂交。在hCG刺激排卵前，在卵泡膜细胞中观察到PAI-1 mRNA的极低表达。给予hCG后，大多数窦状卵泡的卵泡膜细胞中PAI-1 mRNA表达增加，而颗粒细胞中的表达仅限于排卵前卵泡，且仅在基膜解离的区域。在hCG处理前，在卵泡膜细胞中观察到TIMP-1 mRNA的低表达，但在颗粒细胞中未观察到。hCG处理后，无论卵泡大小，卵泡膜细胞中的TIMP-1 mRNA均受到极大刺激，而颗粒细胞中的表达仅限于大的窦状卵泡。本研究证明了PAI-1和TIMP-1 mRNA在LH/hCG刺激的卵巢中的细胞特异性表达，从而通过两种酶及其各自抑制剂的共表达证实了排卵前蛋白水解的局部控制。
