Rosenbruch M
Medical Institute of Environmental Hygiene, Heinrich Heine University Düsseldorf, Germany.
Exp Toxicol Pathol. 1992 Mar;44(1):10-4. doi: 10.1016/S0940-2993(11)80130-2.
Male Wistar rats inhaled amorphous silica (quartz glass VP 203-006) for 12 months. Animals were sacrificed after 4, 8, and 12 months of inhalation and after a post-inhalation period of additional 12 months. Morphological as well as morphometric changes of lung associated lymph nodes are studied in comparison with findings due to crystalline quartz (DQ-12) and with a control group without dust exposure. Qualitatively, the lymph node morphology of the dust exposed groups is the same. During the first months mainly small quartz-typical reaction areas occur. From 12 months severe fibroses are detectable. Relative organ weights and the total lymph node area (LNA) are increased in all dust exposed groups. After inhalation of the amorphous quarz glass, the changes seem to appear with a certain delay. But, at the end of the experiment, the morphometric parameters (quartz-typical areas (QTA) and QTA/LNA-ratio) are more enlarged after the amorphous quartz glass than after crystalline quartz DQ-12. The results show that amorphous as well as crystalline silica can lead to severe lymph node fibrosis. This indicates that occupational exposure limits for amorphous silica are generally justified.
雄性Wistar大鼠吸入无定形二氧化硅（石英玻璃VP 203 - 006）12个月。在吸入4个月、8个月和12个月后以及吸入后额外12个月的观察期后对动物实施安乐死。将肺相关淋巴结的形态学及形态计量学变化与结晶石英（DQ - 12）所致结果以及未接触粉尘的对照组进行比较研究。定性而言，接触粉尘组的淋巴结形态相同。在最初几个月主要出现小的典型石英反应区。从12个月起可检测到严重纤维化。所有接触粉尘组的相对器官重量和淋巴结总面积（LNA）均增加。吸入无定形石英玻璃后，变化似乎出现得有一定延迟。但是，在实验结束时，无定形石英玻璃组的形态计量学参数（典型石英区（QTA）和QTA/LNA比值）比结晶石英DQ - 12组增大得更多。结果表明，无定形和结晶二氧化硅均可导致严重的淋巴结纤维化。这表明对无定形二氧化硅的职业接触限值总体上是合理的。
