Scinicariello F, Rady P, Saltzstein D, Orihuela E, Tyring S K
Department of Microbiology, University of Texas Medical Branch, Galveston, Texas 77550.
Cancer. 1992 Oct 15;70(8):2143-8. doi: 10.1002/1097-0142(19921015)70:8<2143::aid-cncr2820700822>3.0.co;2-3.
Human papillomaviruses (HPV) are among the most common causes of sexually transmitted viral infections in the United States, and HPV types 16, 18, and others have been strongly linked with the development of cervical cancer. DNA from these oncogenic HPV types also has been detected in biopsy specimens of penile intraepithelial and invasive neoplasms, indicating a causal role of these viruses in the malignant transformation of these tissue.
Southern blot analysis and two-dimensional gel electrophoresis were used to investigate the presence and physical state of HPV in a patient with metastatic penile carcinoma.
The presence of HPV 16 DNA integrated into the host's genome was documented in a primary penile squamous cell carcinoma and its lymph node metastasis.
The identical restriction endonuclease cleavage patterns for HPV 16 in both the primary tumor and its lymph node metastasis indicate that both tumors arose from a single clonal event. This finding provides evidence of a causal role of HPV in squamous cell carcinoma of male genitalia.
人乳头瘤病毒（HPV）是美国性传播病毒感染的最常见病因之一，16型、18型及其他HPV类型与宫颈癌的发生密切相关。在阴茎上皮内瘤变和浸润性肿瘤的活检标本中也检测到了这些致癌性HPV类型的DNA，表明这些病毒在这些组织的恶性转化中起因果作用。
采用Southern印迹分析和二维凝胶电泳研究1例转移性阴茎癌患者中HPV的存在情况及其物理状态。
在原发性阴茎鳞状细胞癌及其淋巴结转移灶中均记录到HPV 16 DNA整合到宿主基因组中。
原发性肿瘤及其淋巴结转移灶中HPV 16的限制性内切酶切割模式相同，表明这两个肿瘤起源于单个克隆事件。这一发现为HPV在男性生殖器鳞状细胞癌中的因果作用提供了证据。
