Simms H H, D'Amico R
Department of Surgery, Rhode Island Hospital/Brown University School of Medicine, Providence 02903.
Circ Shock. 1992 Sep;38(1):1-8.
The purpose of this study was to examine how intra-abdominal sepsis and extracellular matrix proteins (fibronectin, laminin) affect adherent polymorphonuclear leukocyte (PMN) function. Two groups of swine were studied: Group I (n = 5) underwent sham laparotomy; Group II (n = 8) underwent cecal ligation and incision. PMN adherent to either fibronectin (F) or laminin (L) had increased candicidal activity over buffer (B) by Group I but not by post-operative day 8 Group II PMN. (Percent specific release 51Cr-Group I--35.00, 68.25, 64.75% for B, F, and L; P less than 0.001 comparing B vs. F or L; Group II--14.25, 12.50, 12.75% for B, F, and L; P = NS comparing B vs. F or L.) To determine the mechanism for this finding, PMN priming was then assessed by evaluating both PMN adherence to extracellular matrix proteins and the cell surface expression of CR1/CR3 by using sheep RBC opsonized with C3b or C3bi. PMN activation was assayed by using MTT-Formazan, myeloperoxidase, and hypochlorous acid (HOCl) production. Fibronectin and laminin increased PMN adherence and CR1/CR3 expression over buffer by Group I and Group II animals. Fibronectin and laminin increased MTT-Formazan, myeloperoxidase, and HOCl production over buffer by Group I PMN but not POD 8 Group II PMN. These results suggest that untreated intra-abdominal sepsis partially abrogates the effect of extracellular matrix proteins on PMN function; in particular, the activation but not priming of adherent PMN by extracellular matrix proteins is reduced in this clinical situation.
本研究的目的是探讨腹腔内脓毒症和细胞外基质蛋白（纤连蛋白、层粘连蛋白）如何影响黏附的多形核白细胞（PMN）功能。研究了两组猪：第一组（n = 5）进行假剖腹手术；第二组（n = 8）进行盲肠结扎和切开。第一组PMN黏附于纤连蛋白（F）或层粘连蛋白（L）时，其杀菌活性相对于缓冲液（B）有所增加，但术后第8天的第二组PMN则没有。（51Cr特异性释放百分比——第一组——B、F和L分别为35.00%、68.25%和64.75%；B与F或L比较，P＜0.001；第二组——B、F和L分别为14.25%、12.50%和12.75%；B与F或L比较，P =无显著性差异）。为了确定这一发现的机制，随后通过评估PMN对细胞外基质蛋白的黏附以及使用用C3b或C3bi调理的绵羊红细胞来评估CR1/CR3的细胞表面表达，来评估PMN的预激。通过使用MTT-甲臜、髓过氧化物酶和次氯酸（HOCl）生成来检测PMN的活化。第一组和第二组动物中，纤连蛋白和层粘连蛋白相对于缓冲液增加了PMN的黏附及CR1/CR3的表达。第一组PMN中，纤连蛋白和层粘连蛋白相对于缓冲液增加了MTT-甲臜、髓过氧化物酶和HOCl的生成，但术后第8天的第二组PMN则没有。这些结果表明，未经治疗的腹腔内脓毒症部分消除了细胞外基质蛋白对PMN功能的影响；特别是，在这种临床情况下，细胞外基质蛋白对黏附的PMN的活化作用降低，但预激作用未受影响。
