Peltoketo H, Isomaa V, Vihko R
Department of Clinical Chemistry, University of Oulu, Finland.
Eur J Biochem. 1992 Oct 1;209(1):459-66. doi: 10.1111/j.1432-1033.1992.tb17310.x.
Genomic 17 beta-hydroxysteroid-dehydrogenase (17-HSD) clones were isolated from a human leucocyte genomic library using cDNA encoding human placental 17-HSD as a probe. The overlapping fragments spanned more than 21 kbp containing the duplications, 6.2 kbp of each, as well as 7 kbp upstream and 1.6 kbp downstream from the duplicated sequences. 17 complete and eight partial Alu elements were clustered in this area, covering about 30% of the region, including the borders of the duplications. Each duplication contained a 17-HSD gene and a conserved region of 1.56 kbp with 98% intercopy similarity. The exon structure of the 17-HSD gene II corresponded to the known cDNA species, but both genes contained a possible promoter region with TATA, GC and inverse CAAT boxes. The 5' flanking regions contained sequences similar to the consensus sequences of cis-acting elements, defined as regulators of 17-HSD gene expression. These putative sequences included estrogen and progesterone/glucocorticoid-response elements and a cyclic-AMP regulatory element.
利用编码人胎盘17β-羟类固醇脱氢酶（17-HSD）的cDNA作为探针，从人白细胞基因组文库中分离出基因组17β-羟类固醇脱氢酶（17-HSD）克隆。重叠片段跨越超过21kbp，包含重复序列，每个重复序列为6.2kbp，以及重复序列上游7kbp和下游1.6kbp。17个完整的和8个部分的Alu元件聚集在该区域，覆盖该区域约30%，包括重复序列的边界。每个重复序列包含一个17-HSD基因和一个1.56kbp的保守区域，拷贝间相似度为98%。17-HSD基因II的外显子结构与已知的cDNA种类相对应，但两个基因都包含一个可能的启动子区域，带有TATA、GC和反向CAAT框。5'侧翼区域包含与顺式作用元件一致序列相似的序列，这些顺式作用元件被定义为17-HSD基因表达的调节因子。这些推定序列包括雌激素和孕酮/糖皮质激素反应元件以及一个环磷酸腺苷调节元件。
