Yoshizawa K, Kiyosawa K, Usuda S, Yabu K, Nakatsuji Y, Yamada S, Furuta K, Tanaka E, Sodeyama T, Furuta S
Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto.
Jpn J Cancer Res. 1992 Aug;83(8):871-7. doi: 10.1111/j.1349-7006.1992.tb01993.x.
A new human hepatocellular carcinoma cell (HCC) line, designated SUHC-1, was derived from a Japanese patient with hepatocellular carcinoma having antibody to hepatitis C virus (HCV) and HCV-RNA in his serum, and established in tissue culture. This cell line exhibited typical epithelial cell morphology in culture as observed by phase-contrast and electron microscopy. The SUHC-1 cells produced albumin and alpha 2-macroglobulin. Chromosomal analysis showed several rearrangements at short and long arms of chromosome 1, 17 and 20 (1p-, 1q-, i(1q), i(17q) and 20q+) with a modal number of 91. HCV-RNA was not detected in the supernatant of SUHC-1 cells by nested polymerase chain reaction assay or in the SUHC-1 cells by the in situ hybridization method. We concluded that complete HCV does not exist in the SUHC-1 cell line. The SUHC-1 cell line is the first line of HCC to have been derived from a patient with persistent HCV infection, and may provide a suitable model for studies of hepatocarcinogenesis related to HCV.
一种新的人肝癌细胞系，命名为SUHC-1，源自一名日本肝癌患者，该患者血清中有丙型肝炎病毒（HCV）抗体和HCV-RNA，并在组织培养中建立。通过相差显微镜和电子显微镜观察，该细胞系在培养中呈现典型的上皮细胞形态。SUHC-1细胞产生白蛋白和α2-巨球蛋白。染色体分析显示，在1号、17号和20号染色体的短臂和长臂上有几种重排（1p-、1q-、i(1q)、i(17q)和20q+），众数为91。通过巢式聚合酶链反应检测，在SUHC-1细胞的上清液中未检测到HCV-RNA，通过原位杂交方法在SUHC-1细胞中也未检测到。我们得出结论，SUHC-1细胞系中不存在完整的HCV。SUHC-1细胞系是首个源自持续性HCV感染患者的肝癌细胞系，可能为研究与HCV相关的肝癌发生提供合适的模型。
