Stevenson D, Lin J H, Tong M J, Marshall G J
Liver Center, Huntington Memorial Hospital, Pasadena, California 91105.
Hepatology. 1987 Nov-Dec;7(6):1291-5. doi: 10.1002/hep.1840070619.
A continuous adherent cell line was established from a hepatocellular carcinoma of an HBsAg-positive Italian male. This cell line, designated Tong/HCC, has been grown in a hormone-supplemented medium for more than 18 months. The cell line secretes HBsAg, alpha-fetoprotein, albumin and alpha 1-antitrypsin. alpha-Fetoprotein production is enhanced by the addition of hydrocortisone and appears to be glucocorticoid concentration-dependent. The concentrates of the supernatant from the cell cultures and cell lysates were negative when tested for HBeAg. The cell culture medium was negative for hepatitis B virus DNA when tested by dot-blot hybridization. However, hepatitis B virus DNA was found to be integrated in the chromosomal DNA by Southern blot analysis. At least five different integration sites were identified, and no free hepatitis B virus DNA was observed. The modal chromosome number was 64, and a translocation on Chromosome 15 was consistently noted. HLA typing revealed sites for A3, Aw24, Bw34 and Cw1.
从一名HBsAg阳性的意大利男性的肝细胞癌中建立了一个连续贴壁细胞系。该细胞系命名为Tong/HCC，已在添加激素的培养基中培养了18个月以上。该细胞系分泌HBsAg、甲胎蛋白、白蛋白和α1-抗胰蛋白酶。添加氢化可的松可增强甲胎蛋白的产生，且似乎呈糖皮质激素浓度依赖性。细胞培养上清液和细胞裂解物的浓缩物在检测HBeAg时呈阴性。通过斑点印迹杂交检测，细胞培养基中的乙型肝炎病毒DNA呈阴性。然而，通过Southern印迹分析发现乙型肝炎病毒DNA整合到了染色体DNA中。至少鉴定出五个不同的整合位点，未观察到游离的乙型肝炎病毒DNA。众数染色体数为64，且始终观察到15号染色体有易位。HLA分型显示有A3、Aw24、Bw34和Cw1位点。
