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在大肠杆菌无细胞系统中，两个不需要EnvZ磷酸化的转录活性OmpR突变体。

Two transcriptionally active OmpR mutants that do not require phosphorylation by EnvZ in an Escherichia coli cell-free system.

作者信息

Bowrin V, Brissette R, Inouye M

机构信息

Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey at Rutgers, Piscataway 08854.

出版信息

J Bacteriol. 1992 Oct;174(20):6685-7. doi: 10.1128/jb.174.20.6685-6687.1992.

DOI:10.1128/jb.174.20.6685-6687.1992
PMID:1328161
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207654/
Abstract

D55Q-T83A and D55Q-G94S, two pseudorevertants of the D55Q mutant OmpR, an Escherichia coli transcriptional activator, were isolated previously by R. Brissette, K. Tsung, and M. Inouye (J. Bacteriol. 173:3749-3755, 1991). These pseudorevertant OmpR proteins were purified and examined for their function as transcriptional activators in a cell-free system with an ompF DNA fragment. These proteins were transcriptionally active, even after acid treatment, whereas the wild-type OmpR was completely inactive after the same treatment. Phosphorylation of acid-treated wild-type OmpR with an EnvZ11 membrane fraction and ATP restored transcriptional activity, whereas the activities of the mutant OmpR proteins did not change after phosphorylation.

摘要

D55Q-T83A和D55Q-G94S是大肠杆菌转录激活因子D55Q突变体OmpR的两个假回复突变体，先前由R. 布里塞特、K. 宗和M. 井上分离得到（《细菌学杂志》173:3749 - 3755, 1991）。这些假回复突变体OmpR蛋白被纯化，并在无细胞体系中用ompF DNA片段检测其作为转录激活因子的功能。这些蛋白即使经过酸处理仍具有转录活性，而野生型OmpR在相同处理后则完全无活性。用EnvZ11膜组分和ATP对酸处理后的野生型OmpR进行磷酸化可恢复转录活性，而突变体OmpR蛋白磷酸化后活性不变。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bda3/207654/7524375be810/jbacter00086-0378-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bda3/207654/1c6abec43c81/jbacter00086-0378-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bda3/207654/9e61408884e6/jbacter00086-0378-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bda3/207654/7524375be810/jbacter00086-0378-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bda3/207654/1c6abec43c81/jbacter00086-0378-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bda3/207654/9e61408884e6/jbacter00086-0378-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bda3/207654/7524375be810/jbacter00086-0378-c.jpg

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1
Two transcriptionally active OmpR mutants that do not require phosphorylation by EnvZ in an Escherichia coli cell-free system.在大肠杆菌无细胞系统中，两个不需要EnvZ磷酸化的转录活性OmpR突变体。
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本文引用的文献

1
Purification and characterization of the OmpR protein, a positive regulator involved in osmoregulatory expression of the ompF and ompC genes in Escherichia coli.大肠杆菌中参与ompF和ompC基因渗透调节表达的正向调节因子OmpR蛋白的纯化与特性分析
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Interaction of OmpR, a positive regulator, with the osmoregulated ompC and ompF genes of Escherichia coli. Studies with wild-type and mutant OmpR proteins.正向调节因子OmpR与大肠杆菌渗透压调节的ompC和ompF基因的相互作用。野生型和突变型OmpR蛋白的研究。
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Localization and membrane topology of EnvZ, a protein involved in osmoregulation of OmpF and OmpC in Escherichia coli.
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Phosphorylation of a bacterial activator protein, OmpR, by a protein kinase, EnvZ, results in stimulation of its DNA-binding ability.蛋白激酶EnvZ对细菌激活蛋白OmpR的磷酸化作用会导致其DNA结合能力增强。
J Biochem. 1989 Jul;106(1):5-7. doi: 10.1093/oxfordjournals.jbchem.a122817.
8
Evidence for the physiological importance of the phosphotransfer between the two regulatory components, EnvZ and OmpR, in osmoregulation in Escherichia coli.关于两种调节成分EnvZ和OmpR之间的磷酸转移在大肠杆菌渗透调节中的生理重要性的证据。
J Biol Chem. 1989 Aug 25;264(24):14090-4.
9
A bacterial environmental sensor that functions as a protein kinase and stimulates transcriptional activation.一种作为蛋白激酶发挥作用并刺激转录激活的细菌环境传感器。
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Transfer of phosphoryl group between two regulatory proteins involved in osmoregulatory expression of the ompF and ompC genes in Escherichia coli.磷酸基团在参与大肠杆菌中ompF和ompC基因渗透调节表达的两种调节蛋白之间的转移。
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