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由痘苗病毒DNA拓扑异构酶I的表达引起的大肠杆菌的条件性生长。

Conditional growth of Escherichia coli caused by expression of vaccinia virus DNA topoisomerase I.

作者信息

Fernandez-Beros M E, Tse-Dinh Y C

机构信息

Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595.

出版信息

J Bacteriol. 1992 Nov;174(21):7059-62. doi: 10.1128/jb.174.21.7059-7062.1992.

DOI:10.1128/jb.174.21.7059-7062.1992
PMID:1328167
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207389/
Abstract

Active vaccinia virus topoisomerase I is expressed in Escherichia coli containing plasmid p1940 (S. Shuman, M. Golder, and B. Moss, J. Biol. Chem. 263:16401-16407, 1988). Growth curves showed a decline of 2 to 3 logs in the number of viable cells at 42 degrees C after shift from 30 degrees C because of increased vaccinia virus topoisomerase I level. Mutations in the gyrA and gyrB genes allowed cells to grow equally well at 42 and 30 degrees C. The presence of gyrase inhibitor also improved growth at 42 degrees C.

摘要

活性痘苗病毒拓扑异构酶I在含有质粒p1940的大肠杆菌中表达（S. 舒曼、M. 戈尔德和B. 莫斯，《生物化学杂志》263:16401 - 16407, 1988年）。生长曲线表明，从30℃转移至42℃后，由于痘苗病毒拓扑异构酶I水平升高，活细胞数量下降了2至3个对数级。gyrA和gyrB基因的突变使细胞在42℃和30℃下生长得同样良好。回旋酶抑制剂的存在也改善了细胞在42℃下的生长情况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46e6/207389/5b83dff4421d/jbacter00087-0364-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46e6/207389/96aca5306d49/jbacter00087-0363-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46e6/207389/e05101617574/jbacter00087-0363-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46e6/207389/5b83dff4421d/jbacter00087-0364-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46e6/207389/96aca5306d49/jbacter00087-0363-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46e6/207389/e05101617574/jbacter00087-0363-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46e6/207389/5b83dff4421d/jbacter00087-0364-a.jpg

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本文引用的文献

1
Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes.大肠杆菌DNA拓扑异构酶I突变体在DNA促旋酶基因中存在补偿性突变。
Cell. 1982 Nov;31(1):43-51. doi: 10.1016/0092-8674(82)90403-2.
2
Escherichia coli DNA topoisomerase I mutants: increased supercoiling is corrected by mutations near gyrase genes.大肠杆菌DNA拓扑异构酶I突变体：超螺旋增加可通过gyrase基因附近的突变得到纠正。
Cell. 1982 Nov;31(1):35-42. doi: 10.1016/0092-8674(82)90402-0.
3
Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and transposition.
Mice lacking DNA topoisomerase IIIbeta develop to maturity but show a reduced mean lifespan.
缺乏DNA拓扑异构酶IIIβ的小鼠能够发育成熟，但平均寿命缩短。
Proc Natl Acad Sci U S A. 2001 May 8;98(10):5717-21. doi: 10.1073/pnas.101132498. Epub 2001 May 1.
编码大肠杆菌DNA拓扑异构酶I的基因发生突变会影响转录和转座。
Proc Natl Acad Sci U S A. 1981 May;78(5):2747-51. doi: 10.1073/pnas.78.5.2747.
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Biology of bacterial deoxyribonucleic acid topoisomerases.细菌脱氧核糖核酸拓扑异构酶的生物学
Microbiol Rev. 1984 Dec;48(4):273-89. doi: 10.1128/mr.48.4.273-289.1984.
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In vitro synthesis of bacteriophage lysozyme.噬菌体溶菌酶的体外合成
Nature. 1967 Aug 5;215(5101):588-91. doi: 10.1038/215588a0.
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A complementation analysis of the restriction and modification of DNA in Escherichia coli.大肠杆菌中DNA限制与修饰的互补分析。
J Mol Biol. 1969 May 14;41(3):459-72. doi: 10.1016/0022-2836(69)90288-5.
7
Consensus sequence for Escherichia coli heat shock gene promoters.大肠杆菌热休克基因启动子的共有序列。
Proc Natl Acad Sci U S A. 1985 May;82(9):2679-83. doi: 10.1073/pnas.82.9.2679.
8
Vaccinia virus encapsidates a novel topoisomerase with the properties of a eucaryotic type I enzyme.痘苗病毒包裹着一种具有真核I型酶特性的新型拓扑异构酶。
J Biol Chem. 1987 Jul 5;262(19):9309-15.
9
Probing the structural domains and function in vivo of Escherichia coli DNA topoisomerase I by mutagenesis.通过诱变探究大肠杆菌DNA拓扑异构酶I的体内结构域和功能
J Mol Biol. 1986 Oct 5;191(3):333-40. doi: 10.1016/0022-2836(86)90130-0.
10
Regulation of the Escherichia coli DNA topoisomerase I gene by DNA supercoiling.DNA超螺旋对大肠杆菌DNA拓扑异构酶I基因的调控
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