Arimori S, Shinozawa S, Hiraki K
Acta Med Okayama. 1975 Dec;29(6):421-9.
An anti-membrane antibody was present in the sera of systemic lupus erythematosus patients in immunoelectrosyneresis with sodium dodecyl sulfate (SDS) solubilized erythrocyte membrane as antigen. The SDS bound to protein was detected by chromatography at 10(-3)M concentration under U.V. light, at 10(-5)M concentration by the distilled water spray method and at 10(-6)M concentration by using rosaniline hydrochloride colorimetry. SDS was removed from the membrane protein at a concentration of 10(-3)M by the first gel filtration of Sephadex G-25 column and at a concentration of 10(-6)M by rechromatography of the same column. More than 99% of SDS in the solubilized erythrocyte membrane was removed by gel filtration. The antigenicity was still positive in the refiltrated fractions of systemic lupus erythematosus patients. Therefore, all precipitates in the gels were antigen-antibody aggregates.
以十二烷基硫酸钠（SDS）溶解的红细胞膜为抗原，通过免疫电渗法发现系统性红斑狼疮患者血清中存在抗膜抗体。通过色谱法在紫外光下以10⁻³M浓度检测与蛋白质结合的SDS，通过蒸馏水喷雾法以10⁻⁵M浓度检测，通过使用盐酸玫瑰苯胺比色法以10⁻⁶M浓度检测。通过Sephadex G - 25柱的第一次凝胶过滤以10⁻³M浓度从膜蛋白中去除SDS，通过同一柱的再色谱法以10⁻⁶M浓度去除。通过凝胶过滤去除了溶解的红细胞膜中99%以上的SDS。系统性红斑狼疮患者的再过滤级分中的抗原性仍为阳性。因此，凝胶中的所有沉淀物都是抗原 - 抗体聚集体。
