Kim H D, Luthra M G, Hildenbrandt G R, Zeidler R B
Am J Physiol. 1976 Jun;230(6):1676-82. doi: 10.1152/ajplegacy.1976.230.6.1676.
A simple centrifugation technique initially developed for fractionation of human red cells in their own plasma has been found to be readily applicable to the separation of reticulocytes normally appearing in the pig soon after birth as well as to those induced in the adult pig by phenylhydrazine administration. The density of the naturally occurring reticulocytes, maximally amounting to 10-15% of total cells derived from a 7-day-old pig, varied from 1.073 to 1.101, in contrast to the mature cells, presumed to be the fetal cell type, whose density ranged in a narrower limit, from 1.101 to 1.106. In adult animals, the reticulocytosis amounting to more than 70% takes place in response to daily administration of phenylhydrazine for 7 days. The density of these reticulocytes varied widely from 1.068 to 1.094; in contrast, the density of the mature adult red cells ranged from 1.095 to 1.106. Among various physiological parameters examined, a marked decrease in cell size, cell water content, RNA, Na+ -K+ -ATPase, and Ca2+ -ATPase activities was observed in the course of reticulocyte maturation in vivo in the adult animal. All these physiological parameters were found to undergo a similar reduction from the top to the bottom fractions of the reticulocytes separated according to their density, suggesting that the separation technique employed here represents separation of reticulocytes according to their age.
一种最初为在人自身血浆中分离人红细胞而开发的简单离心技术，已被发现很容易适用于分离出生后不久正常出现在猪体内的网织红细胞，以及成年猪经苯肼给药诱导产生的网织红细胞。自然存在的网织红细胞密度在1.073至1.101之间，在来自7日龄猪的总细胞中最多占10%-15%，相比之下，推测为胎儿细胞类型的成熟细胞密度范围较窄，在1.101至1.106之间。在成年动物中，每天给予苯肼7天会引发网织红细胞增多，其比例超过70%。这些网织红细胞的密度在1.068至1.094之间变化很大；相比之下，成年成熟红细胞的密度在1.095至1.106之间。在检查的各种生理参数中，成年动物体内网织红细胞在成熟过程中，细胞大小、细胞含水量、RNA、Na+-K+-ATP酶和Ca2+-ATP酶活性均显著降低。根据密度分离的网织红细胞从上到下各部分中，所有这些生理参数都呈现出类似的降低，这表明这里采用的分离技术是根据网织红细胞的年龄进行分离的。
