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通过葡聚糖密度梯度法对出生后前三个月新生儿和婴儿的红细胞进行分离和表征。红细胞和网织红细胞的密度分布曲线。

Separation and characterization of red blood cells from newborns and infants during the first trimenon of life by means of a dextran density gradient. Density distribution curves of erythrocytes and reticulocytes.

作者信息

Schmidt G, Gross J, Grauel E L, Ihle W, Syllm-Rapoport I

出版信息

Biol Neonate. 1977;31(1-2):42-50. doi: 10.1159/000240942.

Abstract

Longitudinal studies on the density of erythrocytes and reticulocytes were performed by means of the isopyknic density gradient centrifugation in dextran on three mature newborns and six premature infants during the first 3 months of life. Striking changes of the density distribution curves of red blood cells took place during the first trimenon. Median density (D50) reached its maximum between the 7th and 28th day of life. D50 of reticulocytes was lower than that of the total red blood cell population and shows similar changes to the whole cell population during the first trimenon after birth. Based on the close relationship between D50 and the percentage of lightest cells, the evaluation of the percentage of the lightest cells was recommended as a simple test to study the changes in median density.

摘要

通过在右旋糖酐中进行等密度密度梯度离心,对3名足月新生儿和6名早产儿出生后前3个月的红细胞和网织红细胞密度进行了纵向研究。在妊娠头三个月期间,红细胞密度分布曲线发生了显著变化。中位密度(D50)在出生后第7天至第28天达到最大值。网织红细胞的D50低于总红细胞群体的D50,并且在出生后的头三个月与整个细胞群体表现出相似的变化。基于D50与最轻细胞百分比之间的密切关系,建议将最轻细胞百分比的评估作为研究中位密度变化的一项简单测试。

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