Manaka H, Kato T, Kurita K, Katagiri T, Shikama Y, Kujirai K, Kawanami T, Suzuki Y, Nihei K, Sasaki H
Third Department of Internal Medicine, Yamagata University School of Medicine, Japan.
Neurosci Lett. 1992 May 11;139(1):47-9. doi: 10.1016/0304-3940(92)90854-z.
We have established the radioimmunoassay for ubiquitin in the cerebrospinal fluid (CSF) and measured the ubiquitin concentration in CSF from 4 cases of neuropathologically verified Creutzfeldt-Jakob disease (CJD), 10 cases of multi-infarct dementia (MID), 7 cases of senile dementia of Alzheimer type (SDAT), and 18 controls. The normal values were determined to range from 7.3 to 21.0 ng/ml, 14.3 +/- 1.1 ng/ml in the mean +/- S.E.M. The CSF ubiquitin levels in the cases of MID and SDAT were 16.6 +/- 6.4 ng/ml and 21.3 +/- 6.1 ng/ml, respectively. In the cases of CJD, the CSF ubiquitin was markedly increased at the early and middle stages of the disease (230.6 ng/ml in Case 1, 107.6 ng/ml in Case 2, 212.5 ng/ml in Case 3, and 377.0 ng/ml in Case 4) and these gradually decreased as the disease progressed. The measurement of CSF ubiquitin seems useful to make an early diagnosis of CJD.
我们已经建立了脑脊液（CSF）中泛素的放射免疫测定法，并测定了4例经神经病理学证实的克雅氏病（CJD）、10例多发性梗死性痴呆（MID）、7例阿尔茨海默型老年性痴呆（SDAT）患者以及18名对照者脑脊液中的泛素浓度。正常范围确定为7.3至21.0纳克/毫升，平均值±标准误为14.3±1.1纳克/毫升。MID和SDAT患者脑脊液中泛素水平分别为16.6±6.4纳克/毫升和21.3±6.1纳克/毫升。在CJD患者中，脑脊液泛素在疾病早期和中期显著升高（病例1为230.6纳克/毫升，病例2为107.6纳克/毫升，病例3为212.5纳克/毫升，病例4为377.0纳克/毫升），并且随着疾病进展逐渐下降。脑脊液泛素的测定似乎有助于CJD的早期诊断。
