使用pET载体提高蛋白质表达的方法。 - Suppr

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超能文献

Methods for improved protein expression using pET vectors.

作者信息

Furlong J, Meighan M, Conner J, Murray J, Clements J B

机构信息

MRC Virology Unit, Glasgow University, UK.

出版信息

Nucleic Acids Res. 1992 Sep 11;20(17):4668. doi: 10.1093/nar/20.17.4668.

DOI:10.1093/nar/20.17.4668

PMID:1329026

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334207/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96d9/334207/44fc9c532806/nar00228-0265-a.jpg

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The unique N-terminal domain of the large subunit of herpes simplex virus ribonucleotide reductase is preferentially sensitive to proteolysis.

J Gen Virol. 1989 Dec;70 ( Pt 12):3159-69. doi: 10.1099/0022-1317-70-12-3159.

Use of T7 RNA polymerase to direct expression of cloned genes.使用T7 RNA聚合酶指导克隆基因的表达。

Methods Enzymol. 1990;185:60-89. doi: 10.1016/0076-6879(90)85008-c.

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