Suzuki K, Suzuki K, Mitsuoka T
Animal and Cellular Systems Laboratory, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Nutr Cancer. 1992;18(1):63-71. doi: 10.1080/01635589209514206.
Normal healthy volunteers (n = 8) received low- and high-fat (14% and 53% energy/day, respectively) and dietary fiber-supplemented high-fat diets (fiber 25 g/day, fat 52% energy/day) for 10 days each. Colon cancer risk factors in feces were measured by colonic nuclear aberration assay, the Ames Salmonella test using strain TA100, and measurement of bile acids and calcium soaps. Nuclear aberrations in colonic epithelium increased during the high-fat diet period and then decreased during the fiber-supplemented high-fat diet period. There were no significant differences in the mutagenicity on Salmonella TA100 or in the concentration of bile acids during the high-fat diet period. Bile acids decreased during the fiber supplementation period. The marked increase in calcium soaps during the high-fat diet period indicates an increase in long-chain fatty acids in the fecal lipid component and conversion of these fatty acids to insoluble calcium soaps when enough calcium is present.
正常健康志愿者（n = 8）分别接受低脂肪和高脂肪饮食（分别为每日能量的14%和53%）以及补充膳食纤维的高脂肪饮食（膳食纤维25克/天，脂肪占每日能量的52%），每种饮食持续10天。通过结肠核畸变试验、使用TA100菌株的艾姆斯沙门氏菌试验以及胆汁酸和钙皂的测量来检测粪便中的结肠癌风险因素。在高脂肪饮食期间，结肠上皮细胞的核畸变增加，而在补充膳食纤维的高脂肪饮食期间则下降。在高脂肪饮食期间，沙门氏菌TA100的致突变性或胆汁酸浓度没有显著差异。在补充膳食纤维期间，胆汁酸减少。高脂肪饮食期间钙皂的显著增加表明粪便脂质成分中长链脂肪酸增加，并且当有足够的钙存在时，这些脂肪酸会转化为不溶性钙皂。
