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A 5' to 3' exonuclease functionally interacts with calf DNA polymerase epsilon.

作者信息

Siegal G, Turchi J J, Myers T W, Bambara R A

机构信息

Department of Biochemistry, University of Rochester School of Medicine and Dentistry, NY 14642.

出版信息

Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9377-81. doi: 10.1073/pnas.89.20.9377.

DOI:10.1073/pnas.89.20.9377
PMID:1329095
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC50134/
Abstract

Analysis of fractions containing purified DNA polymerase epsilon from calf thymus has revealed the presence of a 5' to 3' exonuclease activity that is specific for a single strand of duplex DNA. This activity is capable of degrading a 3'-labeled oligonucleotide hybridized to M13mp18 DNA. When a second oligonucleotide primer is annealed 3 bases upstream, degradation of the downstream primer is strictly dependent on DNA synthesis from the upstream primer. Replacement of the downstream primer by an oligoribonucleotide of identical sequence results in a similar pattern of exonucleolytic activity. The activity has been highly purified and found to cosediment in glycerol gradients with a peptide of 56 kDa as judged by SDS/PAGE analysis. Effects of calf DNA polymerase alpha and delta on exonuclease activity are also observed but with differences in the pattern of products.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a82e/50134/42c2352becb6/pnas01094-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a82e/50134/de25240e9277/pnas01094-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a82e/50134/b71c10513930/pnas01094-0022-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a82e/50134/9421602f427b/pnas01094-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a82e/50134/b0cd1235aaaf/pnas01094-0023-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a82e/50134/42c2352becb6/pnas01094-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a82e/50134/de25240e9277/pnas01094-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a82e/50134/b71c10513930/pnas01094-0022-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a82e/50134/9421602f427b/pnas01094-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a82e/50134/b0cd1235aaaf/pnas01094-0023-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a82e/50134/42c2352becb6/pnas01094-0024-a.jpg

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本文引用的文献

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Selective elimination of the exonuclease activity of the deoxyribonucleic acid polymerase from Escherichia coli B by limited proteolysis.通过有限蛋白酶解选择性去除大肠杆菌B中脱氧核糖核酸聚合酶的核酸外切酶活性。
Proc Natl Acad Sci U S A. 1970 Jan;65(1):168-75. doi: 10.1073/pnas.65.1.168.
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A newly detected class of mammalian single strand-specific DNA-binding proteins. Effects on DNA polymerase alpha-catalyzed DNA synthesis.
FEN1 S187磷酸化在抵抗氧诱导应激及调节出生后心脏发育中的作用。
FASEB J. 2017 Jan;31(1):132-147. doi: 10.1096/fj.201600631R. Epub 2016 Sep 30.
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The replication fork: understanding the eukaryotic replication machinery and the challenges to genome duplication.复制叉：理解真核生物的复制机制以及基因组复制所面临的挑战。
Genes (Basel). 2013 Mar 1;4(1):1-32. doi: 10.3390/genes4010001.
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Fen1 mutations that specifically disrupt its interaction with PCNA cause aneuploidy-associated cancer.Fen1 突变特异性破坏其与 PCNA 的相互作用会导致与非整倍体相关的癌症。
Cell Res. 2011 Jul;21(7):1052-67. doi: 10.1038/cr.2011.35. Epub 2011 Mar 8.
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Functional regulation of FEN1 nuclease and its link to cancer.FEN1 核酸内切酶的功能调控及其与癌症的关联。
Nucleic Acids Res. 2011 Feb;39(3):781-94. doi: 10.1093/nar/gkq884. Epub 2010 Oct 6.
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