Ward A, Hickman D, Gordon J W, Sim E
Department of Pharmacology, University of Oxford, U.K.
Biochem Pharmacol. 1992 Sep 25;44(6):1099-104. doi: 10.1016/0006-2952(92)90373-q.
N-Acetyltransferase activities associated with erythrocytes from 20 individuals have been determined with p-aminobenzoic acid as substrate. A three-fold variation in Vmax is found. The N-acetyltransferase genotype of the individuals has been determined and there is no correlation between the extent of acetylation measured in the individuals' erythrocytes and the inheritance of alleles at the polymorphic NAT locus. Folate is confirmed to be an inhibitor of arylamine N-acetyltransferase activity measured in erythrocytes. The content of folate in erythrocytes of individuals also varies. The individual with the maximum folate content has the minimum N-acetyltransferase activity. The monomorphic N-acetyltransferase gene from individuals spanning the range of N-acetyltransferase activity have been amplified, using the polymerase chain reaction. The pattern of restriction enzyme digestion of the monomorphic N-acetyltransferase gene with a series of eight restriction enzymes is the same for individuals spanning the activity range of arylamine N-acetyltransferase in their erythrocytes.
以对氨基苯甲酸为底物，测定了20名个体红细胞中的N - 乙酰转移酶活性。发现Vmax存在三倍的差异。已确定这些个体的N - 乙酰转移酶基因型，且个体红细胞中测得的乙酰化程度与多态性NAT位点等位基因的遗传之间无相关性。叶酸被确认为红细胞中测得的芳胺N - 乙酰转移酶活性的抑制剂。个体红细胞中的叶酸含量也有所不同。叶酸含量最高的个体N - 乙酰转移酶活性最低。利用聚合酶链反应扩增了N - 乙酰转移酶活性范围各异的个体的单态N - 乙酰转移酶基因。对于红细胞中芳胺N - 乙酰转移酶活性范围各异的个体，用一系列八种限制性内切酶对单态N - 乙酰转移酶基因进行限制性酶切的模式相同。
