Dougherty G, Pasternack R F
Oxconsult, Abingdon, Oxon, England.
Biophys Chem. 1992 Aug;44(1):11-9. doi: 10.1016/0301-4622(92)85031-x.
The base pair selectivity of the intercalative binding of the copper porphyrin, copper (II) tetrakis(4-N-methylpyridyl)porphine (Cu(II)TMpyP-4), to DNA has been investigated using a variety of DNA types and the synthetic polynucleotides poly(dG-dC)2 and poly(dA-dT)2. The studies utilize electron paramagnetic resonance of concentrated gels which are thought to mimic the closely packed state of nuclear DNA. The results indicate that intercalation of this porphyrin is preferred for sites containing two adjacent G-C base pairs, irrespective of sequence.
使用多种DNA类型以及合成多核苷酸聚（dG-dC）2和聚（dA-dT）2，研究了铜卟啉，四（4-N-甲基吡啶基）卟啉铜（II）（Cu（II）TMpyP-4）与DNA嵌入结合的碱基对选择性。这些研究利用了浓缩凝胶的电子顺磁共振，人们认为这种凝胶模拟了核DNA的紧密堆积状态。结果表明，无论序列如何，该卟啉嵌入含有两个相邻G-C碱基对的位点更受青睐。
