来自肝癌细胞系的重组人谷胱甘肽转移酶A1-1的异源表达。
Heterologous expression of recombinant human glutathione transferase A1-1 from a hepatoma cell line.
作者信息
Stenberg G, Björnestedt R, Mannervik B
机构信息
Department of Biochemistry, Uppsala University, Sweden.
出版信息
Protein Expr Purif. 1992 Feb;3(1):80-4. doi: 10.1016/1046-5928(92)90060-a.
A cDNA clone, lambda GTHA1, encoding human glutathione transferase A1-1 has been isolated from a hepatoma HepG2 cDNA library. At the nucleotide level, the new clone showed minor differences from cDNA deriving from normal liver, but the deduced amino acid sequence was identical to the structure previously described. The protein was expressed from a plasmid, pKHA1, and isolated by a single-step affinity purification on an S-hexylglutathione Sepharose matrix. The yield of the recombinant protein was 165 mg from a 3-liter culture of bacteria.
一个编码人谷胱甘肽转移酶A1-1的cDNA克隆，λGTHA1，已从肝癌HepG2 cDNA文库中分离出来。在核苷酸水平上，新克隆与来自正常肝脏的cDNA有微小差异，但推导的氨基酸序列与先前描述的结构相同。该蛋白从质粒pKHA1表达，并通过在S-己基谷胱甘肽琼脂糖基质上进行一步亲和纯化进行分离。从3升细菌培养物中获得的重组蛋白产量为165毫克。
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