不同哺乳动物物种的心脏α1-肾上腺素能受体密度

Cardiac alpha 1-adrenoceptor densities in different mammalian species.

Steinfath M, Chen Y Y, Lavický J, Magnussen O, Nose M, Rosswag S, Schmitz W, Scholz H

Abteilung Allgemeine Pharmakologie, Universitäts-Krankenhaus Eppendorf, Universität Hamburg, Germany.

Br J Pharmacol. 1992 Sep;107(1):185-8. doi: 10.1111/j.1476-5381.1992.tb14484.x.

Abstract

alpha 1-Adrenoceptor densities were studied in cardiac membrane preparations from several mammalian species including human failing hearts under identical experiment conditions; the alpha 1-adrenoceptor antagonist, [3H]-prazosin, was used as radioligand. End-stage heart failure (NYHA IV) in human hearts was due to idiopathic dilated cardiomyopathy. 2. The ventricular alpha 1-adrenoceptor densities were not significantly different in guinea-pig, mouse, pig, calf, and man (11 to 18 fmol mg-1 protein) but about 5 to 8 fold smaller than in rat (about 90 fmol mg-1 protein). Right and left ventricular receptor densities were similar in these species. 3. A sufficient amount of right and left atrial tissue was obtained from rabbit, pig, calf, and man only. The alpha 1-adrenoceptor densities in both atria of these species were found to be at the detection limit of the method used (less than 8 fmol mg-1 protein). 4. The equilibrium dissociation constant (KD) was similar in all species studied ranging from 0.047 +/- 0.006 to 0.063 +/- 0.007 nmol l-1. 5. It is concluded that differences in alpha 1-adrenoceptor density between atria and ventricles may exist in mammalian species. The exceptionally high density of these receptors in rat ventricles seem to be a particular feature in these animals.

摘要

在相同实验条件下，研究了包括人类衰竭心脏在内的几种哺乳动物心脏膜制剂中的α1 - 肾上腺素能受体密度；使用α1 - 肾上腺素能受体拮抗剂[3H] - 哌唑嗪作为放射性配体。人类心脏的终末期心力衰竭（纽约心脏协会IV级）是由特发性扩张型心肌病引起的。2. 豚鼠、小鼠、猪、小牛和人类心室中的α1 - 肾上腺素能受体密度无显著差异（11至18 fmol mg-1蛋白质），但比大鼠（约90 fmol mg-1蛋白质）小约5至8倍。这些物种的右心室和左心室受体密度相似。3. 仅从兔子、猪、小牛和人类获得了足够量的右心房和左心房组织。发现这些物种两个心房中的α1 - 肾上腺素能受体密度处于所用方法的检测限（小于8 fmol mg-1蛋白质）。4. 所有研究物种的平衡解离常数（KD）相似，范围为0.047±0.006至0.063±0.007 nmol l-1。5. 得出结论，哺乳动物物种的心房和心室之间可能存在α1 - 肾上腺素能受体密度差异。大鼠心室中这些受体的异常高密度似乎是这些动物的一个特殊特征。