Joos S, Haluska F G, Falk M H, Henglein B, Hameister H, Croce C M, Bornkamm G W
GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Klinische Molekularbiologie und Tumorgenetik, Munich, Germany.
Cancer Res. 1992 Dec 1;52(23):6547-52.
To analyze the region upstream of c-myc, a number of novel probes were established. These were generated by chromosomal walking starting from the breakpoint of the chromosomal translocation of the B-cell line 380 and by cloning the breakpoint of the translocation of the Burkitt lymphoma cell line IARC/BL72. Using the newly isolated probes a detailed physical map of 500 kilobases of the region upstream of c-myc was established applying pulsed-field gel electrophoresis. The chromosomal breakpoint of IARC/BL72 cells was mapped to a site 55 kilobases 5' of c-myc. A region 20 kilobases in length and containing the breakpoints of 380, EW36, P3HR-1, and Daudi cells was identified 170-190 kilobases upstream of c-myc. In addition the HPV18 integration site in HeLa cells was located between 340 and 500 kilobases 5' of c-myc. The probes were used to define the c-myc amplification units in Colo320-HSR and HL60 cells as well as in four cases of small cell lung cancer. Evidence is provided that the amplicon of HL60 cells is discontinuously organized.
为了分析c-myc上游区域，构建了许多新型探针。这些探针通过从B细胞系380染色体易位的断点开始进行染色体步移以及克隆伯基特淋巴瘤细胞系IARC/BL72易位的断点来产生。使用新分离的探针，通过脉冲场凝胶电泳建立了c-myc上游500千碱基区域的详细物理图谱。IARC/BL72细胞的染色体断点定位于c-myc 5'端55千碱基处。在c-myc上游170 - 190千碱基处鉴定出一个20千碱基长的区域，该区域包含380、EW36、P3HR-1和Daudi细胞的断点。此外，HeLa细胞中HPV18整合位点位于c-myc 5'端340至500千碱基之间。这些探针用于确定Colo320-HSR和HL60细胞以及四例小细胞肺癌中的c-myc扩增单元。有证据表明HL60细胞的扩增子是不连续组织的。
