Reaction intermediates of H-meromyosin-ATPase and ultraviolet difference spectrum of H-meromyosin induced by ATP.
作者信息
Shibata-Sekiya K
出版信息
J Biochem. 1976 Mar;79(3):621-3. doi: 10.1093/oxfordjournals.jbchem.a131105.
Abstract
UV Difference spectra of H-meromyosin (HMM) during the steady state of the myosin-ATPase reaction [EC 3.6.1.3] were measured in 1.5 and 0.05M KC1 in the presence of 5mM MgC1(2) and 20mM Tris-HC1 at pH 8.0 and 24 degrees, using pyruvate kinase [EC 2.7.1.40] and phosphoenolpyruvate to regenerate ATP. It was found that the difference spectrum and its dependence on ATP concentration were the same in 1.5M KC1 as in 0.05M KC1. On the bases of these and other results, the nature of the intermediates of HMM ATPase in the steady-state reaction of HMM ATPase was discussed.
摘要
相似文献
1
Reaction intermediates of H-meromyosin-ATPase and ultraviolet difference spectrum of H-meromyosin induced by ATP.
J Biochem. 1976 Mar;79(3):621-3. doi: 10.1093/oxfordjournals.jbchem.a131105.
3
Reaction mechanism of Mn2+-ATPase of acto-H-meromyosin in 0.1 M KCl at 5 degrees C: evidence for the Lymn-Taylor mechanism.
J Biochem. 1980 Dec;88(6):1653-62. doi: 10.1093/oxfordjournals.jbchem.a133141.
4
Presence of a unit for actin-myosin interaction during the superprecipitation of actomyosin.
J Biochem. 1977 Apr;81(4):1141-6. doi: 10.1093/oxfordjournals.jbchem.a131539.
5
6
Elementary steps in the acto-H-meromyosin ATPase reaction to arterial smooth muscle.
J Biochem. 1978 Aug;84(2):285-92. doi: 10.1093/oxfordjournals.jbchem.a132129.
7
Filamin inhibits actin activation of heavy meromyosin ATPase.
FEBS Lett. 1977 May 15;77(2):228-32. doi: 10.1016/0014-5793(77)80240-8.
8
Separation of subfragment-1 of H-meromyosin into two equimolar fractions with and without formation of the reactive enzyme-phosphate-ADP complex.
J Biochem. 1976 Feb;79(2):419-34. doi: 10.1093/oxfordjournals.jbchem.a131085.
9
Temperature dependence of the decay of the UV absorption difference spectrum of heavy meromyosin induced by adenosine triphosphate and inosine triphosphate.
J Biochem. 1977 Feb;81(2):305-12. doi: 10.1093/oxfordjournals.jbchem.a131459.
10
Desensitization of substrate inhibition of acto-H-meromyosin ATPase by treatment of H-meromyosin with rho-chloromercuribenzoate. Relation between the extent of desensitization and the amount of bound rho-chloromercuribenzoate1.
J Biochem. 1975 Mar;77(3):543-57. doi: 10.1093/oxfordjournals.jbchem.a130755.
Zotero 插件