Yon L, Feuilloley M, Chartrel N, Arimura A, Conlon J M, Fournier A, Vaudry H
European Institute for Peptide Research, Laboratory of Molecular Endocrinology, CNRS URA 650, UA INSERM, University of Rouen, France.
J Comp Neurol. 1992 Oct 22;324(4):485-9. doi: 10.1002/cne.903240403.
The primary structure of frog pituitary adenylate cyclase-activating polypeptide (PACAP) has recently been determined and the results show that the sequence of PACAP has been highly conserved during evolution. In particular, the structure of the 1-27 fragment of PACAP is identical in frog and mammals. Using an antiserum raised against PACAP27, we have investigated the distribution of PACAP-containing neurons in the central nervous system of the frog Rana ridibunda by the immunofluorescence technique. The main populations of immunoreactive perikarya were located in the medial and ventral diencephalon, i.e., the preoptic nucleus, the ventral and dorsal infundibular nuclei, the nucleus posterocentralis thalami, and the ventral and ventrolateral areas of the thalamus. In the telencephalon, sparse cell bodies were found in the nucleus accumbens septi, the amygdala, the pallial commissure, and the bed nucleus of the pallial commissure. In the hindbrain, the torus semicircularis, the nucleus profundus and the nucleus anteroventralis tegmenti of the mesencephalon also contained populations of PACAP-immunoreactive perikarya. Beaded nerve fibers were observed throughout the brain. Occasionally they formed bundles, e.g., from the ventral infundibulum to the external vascular layer of the median eminence, from the central thalamus to the optic tectum, and rostrocaudally, from the nucleus accumbens septi to the nucleus entopeduncularis. Other areas, such as the interpeduncular nucleus, the nucleus isthmi and the roots of cranial nerves V and VIII in the medulla oblongata, were also densely innervated. The adenylate cyclase-stimulating activity of PACAP was tested by using a static incubation technique for hypothalamic slices.(ABSTRACT TRUNCATED AT 250 WORDS)
蛙垂体腺苷酸环化酶激活多肽（PACAP）的一级结构最近已被确定，结果表明PACAP的序列在进化过程中高度保守。特别是，蛙和哺乳动物中PACAP 1-27片段的结构是相同的。我们使用针对PACAP27产生的抗血清，通过免疫荧光技术研究了牛蛙中枢神经系统中含PACAP神经元的分布。免疫反应性胞体的主要群体位于间脑内侧和腹侧，即视前核、腹侧和背侧漏斗核、丘脑后中央核以及丘脑腹侧和腹外侧区域。在端脑，伏隔核、杏仁核、脑皮质连合和脑皮质连合床核中有稀疏的细胞体。在后脑，中脑的半环形隆起、深部核和腹侧前脑盖核也含有PACAP免疫反应性胞体群体。在整个大脑中都观察到了串珠状神经纤维。它们偶尔会形成束，例如从腹侧漏斗到正中隆起的外血管层、从丘脑中央到视顶盖，以及从前向后，从伏隔核到内缰核。其他区域，如脚间核、峡核以及延髓中V和VIII颅神经的根部，也有密集的神经支配。通过对下丘脑切片使用静态孵育技术测试了PACAP的腺苷酸环化酶刺激活性。（摘要截短于250字）
