单纯疱疹病毒IE63在转录后水平发挥作用，以刺激病毒mRNA的3'加工。
Herpes simplex virus IE63 acts at the posttranscriptional level to stimulate viral mRNA 3' processing.
作者信息
McLauchlan J, Phelan A, Loney C, Sandri-Goldin R M, Clements J B
机构信息
Medical Research Council Virology Unit, University of Glasgow, Scotland.
出版信息
J Virol. 1992 Dec;66(12):6939-45. doi: 10.1128/JVI.66.12.6939-6945.1992.
We have shown previously that a novel herpes simplex virus-induced activity, LPF, selectively increases RNA 3'-end processing at the poly(A) site of a late virus gene (J. McLauchlan, S. Simpson, and J. B. Clements, Cell 59:1093-1105, 1989). Here, our in vivo and in vitro analyses both demonstrate that LPF is induced during early stages of virus infection. Studies of virus mutants indicate that expression of the immediate-early IE63 gene is required for induction of this activity. The selective effects on 3' processing displayed in the presence of IE63 provide direct evidence that IE63 can influence this posttranscription process. This extends previous studies which reported increases in reporter gene activity with certain poly(A) sites by IE63 (R. M. Sandri-Goldin and G. E. Mendoza, Genes Dev. 6:848-863, 1992).
我们之前已经表明，一种新型单纯疱疹病毒诱导的活性，即晚期促进因子（LPF），可选择性地增加晚期病毒基因聚腺苷酸化位点处的RNA 3'末端加工（J. 麦克劳克兰、S. 辛普森和J. B. 克莱门茨，《细胞》59:1093 - 1105，1989年）。在此，我们的体内和体外分析均表明，LPF是在病毒感染的早期阶段被诱导产生的。对病毒突变体的研究表明，即刻早期IE63基因的表达是诱导这种活性所必需的。在IE63存在的情况下对3'加工的选择性影响提供了直接证据，证明IE63可以影响这一转录后过程。这扩展了之前的研究，那些研究报道了IE63可使某些聚腺苷酸化位点的报告基因活性增加（R. M. 桑德里 - 戈尔丁和G. E. 门多萨，《基因与发育》6:848 - 863，1992年）。
Zotero 插件