Sonobe H, Manabe Y, Furihata M, Iwata J, Oka T, Ohtsuki Y, Mizobuchi H, Yamamoto H, Kumano O, Abe S
Department of Pathology, Kochi Medical School, Nankoku.
Lab Invest. 1992 Oct;67(4):498-505.
The line of differentiation in synovial sarcoma still remains controversial. Thus far, only a few human synovial sarcoma cell lines have been described. However, their morphologic characteristics have not been fully established.
We established a new synovial sarcoma cell line (HS-SY-II) from pleural effusion with lung metastasis in a typical example of the monophasic spindle cell type. The HS-SY-II cells, in vitro and in vivo, were examined by light microscopy, immunohistochemistry, electron microscopy, and cytogenetics.
The HS-SY-II cells showed a hypertriploid karyotype with complex chromosome abnormalities including pathognomonic t(X;18)(p11;q11), and have been stably maintained for more than 40 months in vitro, showing rather small spindle or polygonal shape without conspicuous pleomorphism. Histologic features of initially and serially transplanted tumors in nude mice were essentially the same as those of the original sarcoma, corresponding to the monophasic spindle cell variant with a prominent palisading pattern and calcified foci in parts. The HS-SY-II cells in vitro and in vivo similarly expressed vimentin and cytokeratin by immunohistochemistry, and also exhibit the same ultrastructural features such as irregularly shaped nuclei with prominent nucleoli, many paranuclearly running intermediate filaments, and filopodia-like processes.
This HS-SY-II cell line retaining the distinct morphological characteristics as the monophasic spindle cell type of synovial sarcoma therefore will be extremely useful for various pathomorphologic investigations on synovial sarcoma.
滑膜肉瘤的分化谱系仍存在争议。迄今为止，仅描述了少数人滑膜肉瘤细胞系。然而，它们的形态学特征尚未完全明确。
我们从一例伴有肺转移的胸腔积液中建立了一种新的滑膜肉瘤细胞系（HS-SY-II），该病例为单相梭形细胞型的典型例子。通过光学显微镜、免疫组织化学、电子显微镜和细胞遗传学对HS-SY-II细胞进行了体外和体内研究。
HS-SY-II细胞显示出超三倍体核型，伴有复杂的染色体异常，包括特征性的t(X;18)(p11;q11)，并已在体外稳定维持超过40个月，呈相当小的梭形或多边形，无明显多形性。裸鼠初始移植瘤和连续传代移植瘤的组织学特征与原始肉瘤基本相同，对应于单相梭形细胞变异型，部分区域有明显的栅栏状结构和钙化灶。通过免疫组织化学，HS-SY-II细胞在体外和体内均同样表达波形蛋白和细胞角蛋白，并且还表现出相同的超微结构特征，如核形状不规则、核仁突出、许多核旁运行的中间丝以及丝状伪足样突起。
这种保留滑膜肉瘤单相梭形细胞型独特形态学特征的HS-SY-II细胞系，因此将对滑膜肉瘤的各种病理形态学研究极为有用。
