Lopez-Gonzalez M A, Guerrero J M, Lucas M
Departamento de Bioquímica Médica y Biología Molecular, Hospital Universitario Virgen Macarena, Facultad de Medicina, Sevilla, Spain.
Life Sci. 1992;51(23):1803-10. doi: 10.1016/0024-3205(92)90051-p.
Phorbol-myristate-acetate (PMA) induced in lymphocytes the production or reactive oxygen intermediates in a process which was stimulated by the presence of vasoactive intestinal peptide (VIP) in a dose-dependent response at VIP concentrations in the range 10(-11)-10(-8) M. The dissociation constant for the high-affinity receptors of VIP agreed with the ID50 of the activation of adenylate cyclase, and the ID50 for the stimulation by VIP of PMA-induced chemiluminescence, which were close to 0.2 nM VIP. Forskolin produced in lymphocytes an effect quite similar to VIP. A comparison of the response to VIP and forskolin of lymphocytes and monocytes showed that, in contrast to forskolin, VIP failed to induce the above described effect in monocytes. A possible mechanism involving protein kinase C, which is activated by PMA, and an intracellular signal linked to VIP receptors is pointed out. This study further supports a role for VIP as a mediator in the neuroimmune system.
佛波醇肉豆蔻酸酯乙酸酯（PMA）在淋巴细胞中诱导活性氧中间体的产生，血管活性肠肽（VIP）的存在以剂量依赖性方式刺激这一过程，VIP浓度范围为10⁻¹¹ - 10⁻⁸ M。VIP高亲和力受体的解离常数与腺苷酸环化酶激活的半数抑制浓度（ID50）一致，以及VIP刺激PMA诱导的化学发光的ID50，均接近0.2 nM VIP。福斯高林在淋巴细胞中产生的效应与VIP非常相似。淋巴细胞和单核细胞对VIP和福斯高林反应的比较表明，与福斯高林不同，VIP未能在单核细胞中诱导上述效应。指出了一种可能涉及被PMA激活的蛋白激酶C以及与VIP受体相关的细胞内信号的机制。本研究进一步支持了VIP作为神经免疫系统中介物的作用。
