Nonomura T, Hatanaka H
Division of Protein Biosynthesis, Osaka University, Japan.
Neurosci Res. 1992 Aug;14(3):226-33. doi: 10.1016/0168-0102(92)90083-o.
We examined the effect of brain-derived neurotrophic factor (BDNF) on cholinergic neurons in culture from postnatal rat basal forebrain by assay of choline acetyltransferase (ChAT) activity and cytochemical staining for acetylcholinesterase (AChE). BDNF was found to increase the ChAT activities but failed to promote the survival of AChE-positive neurons in cultures from neonatal (P3) rats, suggesting that its main role is cholinergic differentiation. In contrast, an enhancement of the survival of AChE-positive neurons and of ChAT activity was observed in cultures from P15-16 rats, suggesting that BDNF's main action is the maintenance of cholinergic neurons. Our results indicate a similarity between BDNF and nerve growth factor effects on the responses of cholinergic neurons of postnatal rat basal forebrain in culture.
我们通过检测胆碱乙酰转移酶（ChAT）活性以及对乙酰胆碱酯酶（AChE）进行细胞化学染色，研究了脑源性神经营养因子（BDNF）对新生大鼠基底前脑培养物中胆碱能神经元的影响。结果发现，BDNF可增加ChAT活性，但未能促进新生（P3）大鼠培养物中AChE阳性神经元的存活，这表明其主要作用是胆碱能分化。相比之下，在P15 - 16大鼠的培养物中，观察到AChE阳性神经元的存活率和ChAT活性均有所增强，这表明BDNF的主要作用是维持胆碱能神经元。我们的结果表明，BDNF与神经生长因子对新生大鼠基底前脑培养物中胆碱能神经元反应的影响具有相似性。
