血小板活化因子是马中性粒细胞和嗜酸性粒细胞体外迁移的介质。

Platelet activating factor is a mediator of equine neutrophil and eosinophil migration in vitro.

作者信息

Foster A P, Lees P, Cunningham F M

机构信息

Department of Veterinary Basic Sciences, Royal Veterinary College, North Mymms, Hertfordshire.

出版信息

Res Vet Sci. 1992 Sep;53(2):223-9. doi: 10.1016/0034-5288(92)90114-h.

DOI:10.1016/0034-5288(92)90114-h

PMID:1332153

Abstract

Platelet activating factor (PAF) is known to be a chemoattractant for equine neutrophils in vivo and in vitro. In this study the in vitro migratory response of equine eosinophils and neutrophils to PAF has been examined and compared with that to leukotriene (LT)B4. PAF (10(-8) to 10(-5) M), but not lyso-PAF (10(-6) M), caused dose related migration of both equine eosinophils and neutrophils, maximal responses occurring at 10(-6) M. Responses to PAF were inhibited by the receptor antagonist WEB 2086. LTB4 (10(-8) to 10(-6) M) also induced migration of both cell types, although the maximum effect was observed with a 10-fold lower concentration. Moreover, the maximum response of equine eosinophils to LTB4 was significantly greater than to PAF. It is concluded that LTB4 and PAF, if released in vivo at sites of allergic or inflammatory reactions, could mediate the recruitment of leucocytes to the involved tissue.

摘要

已知血小板活化因子（PAF）在体内和体外都是马中性粒细胞的趋化因子。在本研究中，已检测了马嗜酸性粒细胞和中性粒细胞对PAF的体外迁移反应，并与它们对白三烯（LT）B4的反应进行了比较。PAF（10^(-8)至10^(-5)M），但溶血PAF（10^(-6)M）不会，引起马嗜酸性粒细胞和中性粒细胞的剂量相关迁移，最大反应出现在10^(-6)M。对PAF的反应被受体拮抗剂WEB 2086抑制。LTB4（10^(-8)至10^(-6)M）也诱导两种细胞类型的迁移，尽管在低10倍的浓度下观察到最大效应。此外，马嗜酸性粒细胞对LTB4的最大反应明显大于对PAF的反应。结论是，如果LTB4和PAF在体内过敏或炎症反应部位释放，它们可能介导白细胞向受累组织的募集。