Zehbe I, Rylander E, Strand A, Wilander E
Department of Pathology and Cytology, University of Uppsala, Sweden.
Anticancer Res. 1992 Sep-Oct;12(5):1383-8.
Human papillomavirus (HPV) detection in biopsies from the lower genital tract may be requested by clinicians as a complement to ordinary histopathological diagnosis. In the present study, two commercial kits, (Enzo Diagnostics Inc., New York, USA and Biohit, Helsinki, Finland) used for in situ hybridization with biotinylated c-DNA probes were compared and the HPV-expression was evaluated in relation to histopathological findings. The Enzo kit identifies HPV-types 6/11, 16/18, 18 and 31/33/51, whereas the Biohit kit has separate probes for HPV 6, 11, 16, 18, 31 and 33, but none for HPV 51. The usefulness of a general probe (probemix) for the visualization of HPV irrespective of type (Enzo Diagnostics Inc.) was also studied Altogether 226 biopsies from the lower female genital tract were formalin-fixed, paraffin-embedded and processed for routine histopathological grading. Consecutive sections were employed for in situ hybridization. 50 biopsies were subject to double-testing with Enzo and Biohit, whereas 176 were tested with Enzo only. Of the double-tested biopsies, 30% displayed a nuclear staining with the Enzo kit and 28% with the Biohit kit. It is concluded that the probes of these two kits have the same sensitivity in detecting HPV in tissue sections. Condylomata acuminata were HPV-positive in 81%, mostly for types 6/11. Flat condylomas were HPV-positive in 35%. The HPV-positivity of biopsies with low grade SIL (I) was 50% and that of high grade SIL (II and III) was 37%. High grade SIL contained either HPV-types 16/18 or 31/33/51. A correlation was found between the occurrence of koilocytosis and the presence of HPV-DNA. HPV-expression was most easily visualized in condylomata acuminata. In epithelium of normal appearance or with inflammatory alterations HPV-DNA was not seen.
临床医生可能会要求对下生殖道活检组织进行人乳头瘤病毒（HPV）检测，作为普通组织病理学诊断的补充。在本研究中，对两种用于与生物素化互补DNA（c-DNA）探针进行原位杂交的商用试剂盒（美国纽约的安佐诊断公司和芬兰赫尔辛基的百得公司）进行了比较，并根据组织病理学结果评估了HPV表达情况。安佐试剂盒可识别HPV-6/11、16/18、18和31/33/51型，而百得试剂盒有针对HPV 6、11、16、18、31和33的单独探针，但没有针对HPV 51的探针。还研究了一种通用探针（探针混合物）（安佐诊断公司）用于可视化HPV（无论何种类型）的效用。总共226份来自女性下生殖道的活检组织用福尔马林固定、石蜡包埋，并进行常规组织病理学分级处理。连续切片用于原位杂交。50份活检组织同时用安佐和百得试剂盒进行检测，而176份仅用安佐试剂盒检测。在同时检测的活检组织中，30%用安佐试剂盒显示核染色，28%用百得试剂盒显示核染色。结论是这两种试剂盒的探针在检测组织切片中的HPV时具有相同灵敏度。尖锐湿疣HPV阳性率为81%，主要为6/11型。扁平湿疣HPV阳性率为35%。低级别鳞状上皮内病变（I级）活检组织的HPV阳性率为50%，高级别鳞状上皮内病变（II级和III级）为37%。高级别鳞状上皮内病变包含HPV-16/18或31/33/51型。发现挖空细胞的出现与HPV-DNA的存在之间存在相关性。HPV表达在尖锐湿疣中最容易可视化。在外观正常或有炎症改变的上皮中未见HPV-DNA。
