Kaplan P, Kucera I
Department of Biochemistry, Faculty of Medicine, Masaryk University, Brno, Czechoslovakia.
Biochem Int. 1992 Oct;28(1):137-42.
Using cultivation at different oxygen tensions, the molar ratio of cardiolipin to haem a in cells of Paracoccus denitrificans was varied systematically from 30 to 300. The molecular activity of cytochrome aa3 (with N, N, N', N'-tetramethyl-p-phenylenediamine as substrate) remained unchanged in this interval, this ruling out any regulatory effect of physiological cardiolipin levels on the terminal oxidase. Titration of anaerobically grown cells with cyanide indicated the presence of cytochrome aa3 which accounted for about 1/4 of the total electron flow from TMPD to oxygen.
通过在不同氧张力下培养，反硝化副球菌细胞中心磷脂与血红素a的摩尔比在30至300之间系统地变化。在此区间内，细胞色素aa3（以N，N，N'，N'-四甲基对苯二胺为底物）的分子活性保持不变，这排除了生理心磷脂水平对末端氧化酶的任何调节作用。用氰化物对厌氧生长的细胞进行滴定表明存在细胞色素aa3，其占从TMPD到氧的总电子流的约1/4。
