Wilkins P C, Dalton H, Podmore I D, Deighton N, Symons M C
Department of Biological Sciences, University of Warwick, Coventry, England.
Eur J Biochem. 1992 Nov 15;210(1):67-72. doi: 10.1111/j.1432-1033.1992.tb17391.x.
The spin-trapping technique has demonstrated that carbon-centered radicals are produced during soluble-methane-monooxygenase catalysis of the hydroxylation of several different types of substrate. The resulting spin-adducts were identified from the hyperfine splitting constants in their EPR spectra. Isotopic labelling showed unequivocally that the trapped radicals were derived from substrate. The carbon-centered substrate radicals are believed to result from hydrogen-atom abstraction by a ferryl species in a cytochrome-P-450-like mechanism. No hydroxy radical nor an oxygen-based radical of any kind was detected in any of the spin-trapping experiments.
自旋捕获技术已证明，在可溶性甲烷单加氧酶催化几种不同类型底物的羟基化反应过程中会产生以碳为中心的自由基。通过电子顺磁共振（EPR）光谱中的超精细分裂常数鉴定出了产生的自旋加合物。同位素标记明确表明捕获的自由基源自底物。据信，以碳为中心的底物自由基是通过类似细胞色素P - 450的机制由铁（IV）氧物种夺取氢原子而产生的。在任何自旋捕获实验中均未检测到羟基自由基或任何种类的氧基自由基。
