Sokolova I A, Volgin A U, Makarova N V, Volgina V V, Shishkin S S, Khodarev N N
National Research Center of Medical Genetics, Russian Academy of Medical Sciences, Moscow.
FEBS Lett. 1992 Nov 30;313(3):295-9. doi: 10.1016/0014-5793(92)81213-6.
The activity of Ca/Mg-dependent endonuclease (CME) is strongly inhibited in myeloma X-63.Ag8.653 and B-hybridoma MLC-1c as compared with mouse splenocytes. Nevertheless, pronounced internucleosomal chromatin degradation occurs in both cell lines during long-term cultivation without passing. In isolated cell nuclei of X-63 the activation of CME, which precedes chromatin fragmentation in vivo and loss of cell viability, is revealed. The time-course of CME activation is opposite to cell proliferation and is not accompanied by alterations in enzyme quantity. The results suggest that cell death of X-63 and MLC-1c occurs via apoptosis, and involves the mechanisms controlling the activation and/or interaction of CME with chromatin.
