转座子Tn501杂交用于检测海洋环境中的汞抗性序列。

Hybridization of transposon Tn501 for detection of mercury resistance sequences in a marine environment.

作者信息

Prabu S K, Mahadevan A

机构信息

Centre for Advanced Study in Botany, University of Madras, India.

出版信息

Res Microbiol. 1992 Mar-Apr;143(3):341-5. doi: 10.1016/0923-2508(92)90026-k.

DOI:10.1016/0923-2508(92)90026-k

PMID:1333090

Abstract

Total genomic DNA isolated by concentrating seawater and mercury-resistant bacteria were hybridized with a mer probe to detect the presence of homologous DNA sequences in marine coastal waters of the Bay of Bengal, India. Coastal water extracts induced with mercury hybridized with the mer operon of transposon Tn501. Most of the mercury-resistant bacteria that volatilized mercury also contained homologous DNA sequences to the mer probe.

摘要

通过浓缩海水分离得到的总基因组DNA以及耐汞细菌与汞抗性基因探针（mer探针）杂交，以检测印度孟加拉湾沿海水域中同源DNA序列的存在。用汞诱导的沿海水提取物与转座子Tn501的mer操纵子杂交。大多数挥发汞的耐汞细菌也含有与mer探针同源的DNA序列。