Mashima K, Mizuguchi J
Laboratory of Biochemistry, College of Science, Rikkyo (St. Paul's) University, Tokyo, Japan.
Biochem Biophys Res Commun. 1992 Nov 30;189(1):350-5. doi: 10.1016/0006-291x(92)91565-8.
BRL, a non-malignant rat liver epithelial-like cell line, possessed the ability to adhere through fibronectin to a solid substrate. Oncogenical transformation of these BRL cells with RSV induced a significant decrease in the fibronectin molecules in the extracellular matrix and reduction in its ability to adhere to fibronectin. The alpha 5 and beta 1 subunits of integrin (fibronectin receptor) were quantitatively diminished during RSV transformation in BRL cells. These results suggest that adhesive reduction of BRL cells to a substrate by RSV transformation may be caused by a decrease in cell surface fibronectin and fibronectin receptor molecules.
BRL是一种非恶性大鼠肝上皮样细胞系，具有通过纤连蛋白粘附到固体基质上的能力。用劳斯肉瘤病毒（RSV）对这些BRL细胞进行致癌转化，导致细胞外基质中纤连蛋白分子显著减少，其粘附纤连蛋白的能力降低。在BRL细胞的RSV转化过程中，整合素（纤连蛋白受体）的α5和β1亚基数量减少。这些结果表明，RSV转化导致BRL细胞对基质的粘附减少可能是由于细胞表面纤连蛋白和纤连蛋白受体分子减少所致。
