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加热对脊髓灰质炎补体结合抗原同型特异性的改变。

Modification of the homotypic specificity of poliomyelitis complement-fixing antigens by heat.

作者信息

SCHMIDT N J, LENNETTE E H

出版信息

J Exp Med. 1956 Jul 1;104(1):99-120. doi: 10.1084/jem.104.1.99.

DOI:10.1084/jem.104.1.99
PMID:13332183
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2136636/
Abstract

Fluid obtained from HeLa cell cultures infected with poliomyelitis viruses served as a complement-fixing antigen. When used in the native state, i.e. untreated in any way, the fluids acted as homotypically specific antigens. When heated, however, the antigenicity was broadened and a high degree of heterotypic reactivity was encountered. Data are presented indicating that the observed group reactivity was apparently based on common antigens shared by the three immunologic types of poliomyelitis virus. This reactivity appeared to be specific for the poliomyelitis viruses. No evidence was obtained in preliminary experiments that heating of the antigens releases a "soluble" antigen responsible for the group reactivity.

摘要

从感染脊髓灰质炎病毒的海拉细胞培养物中获取的液体用作补体结合抗原。当以天然状态使用时，即未经任何处理，这些液体作为同型特异性抗原起作用。然而，加热后，抗原性会变宽，会出现高度的异型反应性。所呈现的数据表明，观察到的组反应性显然是基于脊髓灰质炎病毒三种免疫类型共有的共同抗原。这种反应性似乎对脊髓灰质炎病毒具有特异性。在初步实验中没有获得证据表明抗原加热会释放出负责组反应性的“可溶性”抗原。

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本文引用的文献

1
Transitory appearance of type 2 neutralizing antibody in patients infected with type 1 poliomyelitis virus.1型脊髓灰质炎病毒感染患者中2型中和抗体的短暂出现。
J Exp Med. 1952 Jul;96(1):99-106. doi: 10.1084/jem.96.1.99.
2
Complement fixation with Brunhilde and Lansing poliomyelitis viruses propagated in tissue culture.用在组织培养中繁殖的布伦希尔德株和兰辛株脊髓灰质炎病毒进行补体结合试验。
Proc Soc Exp Biol Med. 1952 Feb;79(2):296-300. doi: 10.3181/00379727-79-19357.
3
Tissue culture diagnosis of poliomyelitis and aseptic meningitis.脊髓灰质炎和无菌性脑膜炎的组织培养诊断
J Am Med Assoc. 1955 Jul 2;158(9):707-12. doi: 10.1001/jama.1955.02960090001001.
4
A simplified colorimetric test for poliomyelitis virus and antibody.一种用于检测脊髓灰质炎病毒和抗体的简化比色试验。
Proc Soc Exp Biol Med. 1955 Jan;88(1):114-8. doi: 10.3181/00379727-88-21508.
5
Isolation of virus and the development of neutralizing and complement-fixing antibodies in poliomyelitic patients.脊髓灰质炎患者病毒的分离及中和抗体与补体结合抗体的产生
Ann N Y Acad Sci. 1955 Sep 27;61(4):1005-10. doi: 10.1111/j.1749-6632.1955.tb42559.x.
6
Poliomyelitis complement-fixing antibodies and their demonstration.脊髓灰质炎补体结合抗体及其检测
Ann N Y Acad Sci. 1955 Sep 27;61(4):781-6; discussion, 786-9. doi: 10.1111/j.1749-6632.1955.tb42534.x.
7
Appearance of soluble and cross-reactive complement-fixing antigens on treatment of poliovirus with formalin.用福尔马林处理脊髓灰质炎病毒后可溶性及交叉反应性补体结合抗原的出现。
Proc Soc Exp Biol Med. 1955 Jul;89(3):353-5. doi: 10.3181/00379727-89-21808.
8
A complement fixation test for poliomyelitis.一项针对脊髓灰质炎的补体结合试验。
J Exp Med. 1955 Aug 1;102(2):133-50. doi: 10.1084/jem.102.2.133.
9
[Methods of virus diagnosis in poliomyelitis].[脊髓灰质炎病毒诊断方法]
Nord Med. 1954 Dec 2;52(49):1686-9.
10
Use of color change of phenol red as the indicator in titrating poliomyelitis virus or its antibody in a tissue-culture system.在组织培养系统中，使用酚红颜色变化作为滴定脊髓灰质炎病毒或其抗体的指示剂。
Am J Hyg. 1954 Sep;60(2):214-30. doi: 10.1093/oxfordjournals.aje.a119714.