研究笔记：火鸡顶体蛋白酶的动力学及抑制研究

Research note: kinetic and inhibition studies with turkey acrosin.

作者信息

Richardson M E, Korn N, Bodine A B, Thurston R J

机构信息

Department of Animal, Dairy, and Veterinary Sciences, Clemson University, South Carolina 29634.

出版信息

Poult Sci. 1992 Oct;71(10):1789-93. doi: 10.3382/ps.0711789.

DOI:10.3382/ps.0711789

PMID:1333593

Abstract

Acrosin was extracted from turkey spermatozoa and partially purified by chromatofocusing. Enzyme activity was tested over a pH range with three different substrates. In each case, the pH optimum was between pH 8 and 9. When N-alpha-benzoyl-DL-arginine-p-nitroanilide.HCl (BAPNA) was used as a substrate, the Km and Vmax were 1.17 +/- .05 x 10(-3) M and 1.50 +/- .07 x 10(4) mumol/min.milligram, respectively. Turkey acrosin amidase activity was inhibited by aprotinin, ovomucoid, soybean trypsin inhibitor, benzamidine, p-aminobenzamidine, and zinc.

摘要

从火鸡精子中提取顶体蛋白酶，并通过色谱聚焦法进行部分纯化。使用三种不同的底物在不同pH范围内测试酶活性。在每种情况下，最适pH值在8至9之间。当使用N-α-苯甲酰-DL-精氨酸对硝基苯胺盐酸盐（BAPNA）作为底物时，米氏常数（Km）和最大反应速度（Vmax）分别为1.17±0.05×10⁻³M和1.50±0.07×10⁴μmol/分钟·毫克。火鸡顶体蛋白酶的酰胺酶活性受到抑肽酶、卵类粘蛋白、大豆胰蛋白酶抑制剂、苯甲脒、对氨基苯甲脒和锌的抑制。