[大鼠和人脑中使君子氨酸受体的药理学特性、免疫化学鉴定及定位研究]
[The pharmacological characteristics, immunochemical identification and study of the location of quisqualate receptors in the rat and human brains].
作者信息
Dambinova S A, Bobryshev Iu V, Gorodinskiĭ A I, Margulis M N, Nikitin A O, Orlova E A, Pavlov I Iu, Savinov A Iu
出版信息
Zh Evol Biokhim Fiziol. 1992 Mar-Apr;28(2):201-10.
The kinetics of [3H]-L-glutamate binding to brain synaptic membranes (SM) and to glutamate-binding proteins (GBP) was determined with agonist and monoclonal antibodies (MAbs). It was revealed, that rat and human brain GBP have individual protein components with M(r) from 14 to 92 kDa. Quisqualate inhibited [3H]-L-glutamate binding to solubilized and to purified 68 kDa protein component. MAbs have the most activity, and NMDA was failure. It has been shown that 68 kDa component antigen determinants are similar to those of bovine, frog and rat brain synaptic membranes. Anti-GBP monoclonal antibodies blocked functional non-NMDA receptors in isolated frog spinal cord. Immunocytochemistry was done on rat and human brain sections. Distribution of quisqualate receptors was determined with light and electron microscopy. Some properties of vertebrate CNS non-NMDA receptors are discussed.
用激动剂和单克隆抗体（MAbs）测定了[3H]-L-谷氨酸与脑突触膜（SM）及谷氨酸结合蛋白（GBP）的结合动力学。结果表明，大鼠和人脑GBP具有分子量在14至92 kDa之间的单个蛋白质成分。quisqualate抑制[3H]-L-谷氨酸与溶解的和纯化的68 kDa蛋白质成分的结合。单克隆抗体活性最强，而NMDA则无效。已表明68 kDa成分的抗原决定簇与牛、蛙和大鼠脑突触膜的相似。抗GBP单克隆抗体阻断了离体蛙脊髓中的功能性非NMDA受体。对大鼠和人脑切片进行了免疫细胞化学研究。用光镜和电镜确定了quisqualate受体的分布。讨论了脊椎动物中枢神经系统非NMDA受体的一些特性。
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