Aharonovits O, Zik M, Livne A A, Granot Y
Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Biochim Biophys Acta. 1992 Dec 9;1112(2):181-6. doi: 10.1016/0005-2736(92)90390-8.
The regulation of intracellular Na+ and pHi in human blood platelets is known to be controlled by the function of the Na+/H+ exchanger. The phosphorylation state of the Na+/H+ exchanger which determines the exchanger activity in human blood platelets is regulated by the activities of protein kinases and protein phosphatases. Observations in this study indicate that arginine vasopressin (AVP) that interacts with a V1 receptor, activates the Na+/H+ exchange in human blood platelets through a genistein-inhibited mechanism. The AVP-activated Na+/H+ exchange is probably not regulated by protein kinase C (PKC), since this activation is not inhibited by staurosporine. The multiple ways in which platelet Na+/H+ exchange can be modulated may indicate the critical role played by this exchanger in the homeostasis control of pHi in human blood platelets.
已知人血小板中细胞内钠离子（Na⁺）和细胞内pH值（pHi）的调节受Na⁺/H⁺交换体功能的控制。决定人血小板中交换体活性的Na⁺/H⁺交换体的磷酸化状态受蛋白激酶和蛋白磷酸酶活性的调节。本研究中的观察结果表明，与V1受体相互作用的精氨酸加压素（AVP）通过一种受染料木黄酮抑制的机制激活人血小板中的Na⁺/H⁺交换。AVP激活的Na⁺/H⁺交换可能不受蛋白激酶C（PKC）调节，因为这种激活不受星形孢菌素抑制。血小板Na⁺/H⁺交换的多种调节方式可能表明该交换体在人血小板pHi的稳态控制中发挥的关键作用。
