Isolation of amine oxidase from bovine plasma by a two-step procedure.
作者信息
Vianello F, Di Paolo M L, Zennaro L, Stevanato R, Rigo A
机构信息
Department of Biological Chemistry, University of Padova, Italy.
出版信息
Protein Expr Purif. 1992 Oct;3(5):362-7. doi: 10.1016/s1046-5928(05)80036-1.
PMID:1333859
Abstract
A novel method for isolation of amine oxidase from bovine plasma is reported; it involves a two-step procedure, namely ammonium sulfate fractionation and affinity chromatography with elution by aniline, which is a competitive inhibitor of the enzyme. A homogeneous enzyme, characterized by a specific activity of 0.44 U/mg, was obtained with a yield higher than 50%.
摘要
Zotero 插件