Penschow J D, Coghlan J P
Howard Florey Institute of Experimental Physiology and Medicine, Parkville, Vic., Australia.
Mol Cell Endocrinol. 1992 Oct;88(1-3):23-30. doi: 10.1016/0303-7207(92)90005-q.
As part of an ongoing study of the cell-specific expression of glandular kallikrein genes in mice, we have investigated cellular sites of expression of the renal/pancreatic kallikrein gene, mGK-6, during fetal life. Expression of alpha I and beta-subunit genes of Na+K+ATPase and bradykinin binding were used as an indication of the functional maturity of the fetal epithelial tubules in which mGK-6 expression was identified. mGK-6 mRNA was first observed at embryonic day 16 (E16) in the submandibular main duct, then at E18 in the sub-lingual main duct, at E19 in renal tubules and at E19 in ducts of the nasal glands. All of these ducts contained detectable epithelial Na+K+ATPase mRNAs from an earlier gestational age than mGK-6 mRNA, suggesting their capacity for electrolyte transport. Bradykinin binding was evident in renal tubules at E18. This study established that renal/pancreatic kallikrein is synthesized in fetal epithelial tubules which are mature functionally.
作为一项正在进行的关于小鼠腺激肽释放酶基因细胞特异性表达研究的一部分，我们研究了胎儿期肾/胰激肽释放酶基因mGK-6的细胞表达位点。钠钾ATP酶的αI和β亚基基因的表达以及缓激肽结合被用作胎儿上皮小管功能成熟的指标，在这些小管中鉴定出了mGK-6的表达。mGK-6 mRNA首先在胚胎第16天（E16）在下颌下主导管中被观察到，然后在E18时在舌下主导管中被观察到，在E19时在肾小管中被观察到，在E19时在鼻腺导管中被观察到。所有这些导管在比mGK-6 mRNA更早的胎龄时就含有可检测到的上皮钠钾ATP酶mRNA，表明它们具有电解质转运能力。缓激肽结合在E18时在肾小管中很明显。这项研究确定肾/胰激肽释放酶是在功能成熟的胎儿上皮小管中合成的。
