鳗鱼肠道中缓激肽释放酶原受体的特性研究
Characterization of a kallidin receptor in the eel intestine.
作者信息
Cougnon N, Deacon C F, Lilley K S, Henderson I W
机构信息
Department of Animal and Plant Sciences, Sheffield University, UK.
出版信息
Agents Actions Suppl. 1992;38 ( Pt 2):382-9.
Edman degradation of an eel bradykinin (BK) -like peptide isolated and detected by gel filtration and HPLC and RIA gave an amino acid sequence of Arg1-Pro-Pro-Gly-X-Ser-Pro-Leu-Arg9. Kallidin but not BK and des-Arg9-BK contracted eel intestine. The contractile effect of kallidin was not decreased by B1 and B2 receptor antagonists (up to 10(-6)M), nor by anticholinergics, antiadrenergics, ganglion blockers and an angiotensin II receptor antagonist but was attenuated by 10(-5)M indomethacin. Kallidin appears to interact with a receptor different from the BK B1 and B2 receptor types and prostaglandins may participate in the response.
通过凝胶过滤、高效液相色谱法（HPLC）和放射免疫分析（RIA）分离并检测到的一种鳗鱼缓激肽（BK）样肽的埃德曼降解法给出了氨基酸序列：精氨酸1-脯氨酸-脯氨酸-甘氨酸-X-丝氨酸-脯氨酸-亮氨酸-精氨酸9。胰激肽能使鳗鱼肠道收缩，但缓激肽和去-精氨酸9-缓激肽不能。B1和B2受体拮抗剂（高达10^(-6)M）、抗胆碱能药、抗肾上腺素能药、神经节阻滞剂和血管紧张素II受体拮抗剂均不能降低胰激肽的收缩作用，但10^(-5)M的吲哚美辛可使其作用减弱。胰激肽似乎与不同于BK B1和B2受体类型的受体相互作用，前列腺素可能参与了该反应。
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