Yoshikawa T, Takano H, Naito Y, Oyamada H, Ueda S, Kondo M
First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
Int J Immunopharmacol. 1992 Nov;14(8):1391-8. doi: 10.1016/0192-0561(92)90010-i.
We investigated the effects of tumor necrosis factor-alpha (human, natural type: n-TNF) on polymorphonuclear leukocyte (PMN)-derived superoxide generation by the new method of Cypridina luciferin analog-dependent chemiluminescence, which had high sensitivity and specificity to superoxide. Preincubation of PMNs with n-TNF for 3 min increased PMN-derived superoxide generation induced by phorbol myristate acetate, A23187, opsonized zymosan and N-formyl-methionyl-leucyl-phenylalanine in a concentration dependent manner (0.5-50 Japan reference units/ml of n-TNF). In addition, the enhanced PMN-derived superoxide generation by n-TNF showed a positive correlation to the preincubation time of PMNs with n-TNF (3-15 min). However, a direct incubation of PMNs with n-TNF for 1 h did not induce superoxide from PMNs without the above stimulants. The augmentative effects of n-TNF on PMN-derived superoxide generation should be useful in the PMN-mediated host defense mechanism, such as bactericidal and antitumor activity. The local concentration of n-TNF and the n-TNF-PMN contact time are considered very important in obtaining these effects more efficiently in addition to the presence of PMN-stimulants including complements, chemotactic peptides and phorbol esters.
我们采用对超氧化物具有高灵敏度和特异性的海萤荧光素类似物依赖性化学发光新方法，研究了肿瘤坏死因子-α（人，天然型：n-TNF）对多形核白细胞（PMN）衍生的超氧化物生成的影响。用n-TNF预孵育PMN 3分钟，可使佛波醇肉豆蔻酸酯乙酸盐、A23187、调理酵母聚糖和N-甲酰甲硫氨酰亮氨酰苯丙氨酸诱导的PMN衍生超氧化物生成呈浓度依赖性增加（n-TNF浓度为0.5 - 50日本参考单位/毫升）。此外，n-TNF增强的PMN衍生超氧化物生成与PMN用n-TNF预孵育时间（3 - 15分钟）呈正相关。然而，在没有上述刺激物的情况下，将PMN与n-TNF直接孵育1小时并不会诱导PMN产生超氧化物。n-TNF对PMN衍生超氧化物生成的增强作用在PMN介导的宿主防御机制中应是有用的，如杀菌和抗肿瘤活性。除了存在包括补体、趋化肽和佛波酯在内的PMN刺激物外，n-TNF的局部浓度和n-TNF与PMN的接触时间对于更有效地获得这些效果也非常重要。
