血小板活化因子（PAF）在人多形核白细胞产生超氧化物中的作用。
Role of platelet-activating factor (PAF) in superoxide production by human polymorphonuclear leukocytes.
作者信息
Takahashi S, Yoshikawa T, Naito Y, Tanigawa T, Yoshida N, Kondo M
机构信息
First Department of Medicine, Kyoto Prefectural University of Medicine, Japan.
出版信息
Lipids. 1991 Dec;26(12):1227-30. doi: 10.1007/BF02536537.
The effect of platelet-activating factor (PAF) in superoxide production by human polymorphonuclear leukocytes (PMN) was studied. Cypridina luciferin analog (CLA) dependent chemiluminescence was used to detect superoxide anion radicals. PAF induced superoxide generation in human PMN in a dose-dependent manner. Preincubation with a small amount of PAF (5 x 10(-9) M) enhanced PMN superoxide release induced by various stimuli, such as phorbol myristate acetate (PMA), opsonized zymosan (OZ), calcium ionophore (A23187) and N-formyl-methionyl-leucyl-phenylalanine (FMLP). The PAF antagonist, CV-6209, inhibited superoxide production induced by PAF, but not that induced by other stimuli. These findings would indicate that PAF may play an important role at inflammatory reaction sites and that CV-6209 may inhibit excessive inflammatory reaction.
研究了血小板活化因子（PAF）对人多形核白细胞（PMN）产生超氧化物的影响。采用海萤荧光素类似物（CLA）依赖的化学发光法检测超氧阴离子自由基。PAF以剂量依赖的方式诱导人PMN产生超氧化物。用少量PAF（5×10⁻⁹ M）预孵育可增强由多种刺激物诱导的PMN超氧化物释放，这些刺激物包括佛波酯肉豆蔻酸乙酸酯（PMA）、调理酵母聚糖（OZ）、钙离子载体（A23187）和N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）。PAF拮抗剂CV-6209可抑制PAF诱导的超氧化物产生，但不抑制其他刺激物诱导的超氧化物产生。这些发现表明PAF可能在炎症反应部位起重要作用，且CV-6209可能抑制过度的炎症反应。
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