Messina L, Spampinato G, Arcidiacono A, Malaguarnera L, Pagano M, Kaminska B, Kaczmarek L, Messina A
Institute of General Pathology, University of Catania, Italy.
J Leukoc Biol. 1992 Dec;52(6):585-7. doi: 10.1002/jlb.52.6.585.
Polyamines naturally occur in all living beings and play an important role in the regulation of cell proliferation, differentiation, and functional stimulation of terminally differentiated cells. Our studies, using specific inhibitors of polyamine biosynthesis such as alpha-difluoromethylornithine and methylglyoxal-bis[guanylhydrazone] to prevent polyamine accumulation, have indicated that polyamines are associated with functional activation of human macrophages. Both inhibitors diminished the respiratory burst activity of macrophages induced by lipopolysaccharide and interferon gamma. The methylglyoxal-bis]guanylhydrazone] inhibitory effect was concentration-dependent and could be reversed by spermine, which is the final product of polyamine biosynthesis.
多胺天然存在于所有生物中，在调节细胞增殖、分化以及终末分化细胞的功能刺激方面发挥着重要作用。我们的研究使用了多胺生物合成的特异性抑制剂，如α-二氟甲基鸟氨酸和甲基乙二醛双[脒腙]来防止多胺积累，结果表明多胺与人类巨噬细胞的功能激活有关。这两种抑制剂都降低了脂多糖和干扰素γ诱导的巨噬细胞呼吸爆发活性。甲基乙二醛双[脒腙]的抑制作用呈浓度依赖性，并且可以被精胺逆转，精胺是多胺生物合成的终产物。
