Ducancel F, Gillet D, Ménez A, Boulain J C
Département d'Ingénierie et d'Etudes des Protéines, C.E.A. Saclay, Gif-sur-Yvette.
C R Acad Sci III. 1992;315(6):221-4.
We constructed an expression vector comprising a transcription unit composed of (1) the gene of alkaline phosphatase from E. coli in which we inserted a DNA fragment encoding the VH and CH domains of an IgG2A; (2) a DNA fragment encoding the light chain of the same immunoglobulin. Bacteria, E. coli were transformed with the plasmid, and hence it produced a periplasmic and chimaeric protein having both the alkaline phosphatase enzymatic activity and the immunoglobulin binding function. The hybrid protein mimics a bivalent antibody whose Fc part is replaced by a dimer of alkaline phosphatase. Recombinant colorimetric antibodies offer interesting potentialities for future developments in the field of immuno-enzymatic diagnosis.
我们构建了一个表达载体，其包含一个转录单元，该转录单元由以下部分组成：（1）来自大肠杆菌的碱性磷酸酶基因，我们在其中插入了一个编码IgG2A的VH和CH结构域的DNA片段；（2）一个编码相同免疫球蛋白轻链的DNA片段。用该质粒转化大肠杆菌，因此它产生了一种具有碱性磷酸酶酶活性和免疫球蛋白结合功能的周质嵌合蛋白。该杂合蛋白模拟了一种二价抗体，其Fc部分被碱性磷酸酶二聚体所取代。重组比色抗体为免疫酶诊断领域的未来发展提供了有趣的潜力。
