Malaisse W J
Laboratory of Experimental Medicine, Brussels Free University, Belgium.
Diabetes Res. 1992 Jan;19(1):17-20.
When either rat or human erythrocytes were incubated for 90-180 min in bicarbonate-buffered media prepared in 2H2O rather than 1H2O, the generation of 3HOH from D-[5-3H]glucose, the production of either 14CO2 or 14C-labelled acidic metabolites from D-[U-14C]glucose and the production of L-lactic acid from unlabelled D-glucose were decreased, to a variable extent but, in no case, by more than 30%. Hence, total substitution of 1H2O by 2H2O provides a suitable tool to study by NMR the generation of 2H-enriched L-lactic acid generated from exogenous D-[1-13C]glucose or D-[6-13C]glucose and, hence, to further explore the diabetes-induced alteration of hydrogen isotopes intermolecular transfer in the reaction catalyzed by phosphoglucoisomerase.
当大鼠或人类红细胞在以重水（2H₂O）而非普通水（1H₂O）配制的碳酸氢盐缓冲介质中孵育90 - 180分钟时，D-[5-³H]葡萄糖生成³HOH、D-[U-¹⁴C]葡萄糖生成¹⁴CO₂或¹⁴C标记的酸性代谢产物以及未标记的D-葡萄糖生成L-乳酸的量均有不同程度减少，但在任何情况下减少幅度均不超过30%。因此，用重水完全替代普通水为通过核磁共振（NMR）研究由外源性D-[1-¹³C]葡萄糖或D-[6-¹³C]葡萄糖生成富含²H的L-乳酸提供了合适的工具，从而进一步探究糖尿病引起的磷酸葡萄糖异构酶催化反应中氢同位素分子间转移的改变。
