Bianco P, Haladjian J, Bruschi M, Guerlesquin F
Laboratoire de Chimie et Electrochimie des Complexes, C.N.R.S., Université de Provence, Marseille, France.
Biochem Biophys Res Commun. 1992 Dec 15;189(2):633-9. doi: 10.1016/0006-291x(92)92247-u.
In order to understand the electron transfer mechanisms for the [Fe] and [Ni-Fe] hydrogenases, a kinetic study of cytochrome c3 reduction has been undertaken. Cyclic voltammetry and controlled-potential amperometry techniques have been used to investigate the intermolecular electron-transfer reaction between cytochrome c3 and [Fe] hydrogenase from Desulfovibrio vulgaris Hildenborough. Electron-transfer cross-reactions between [Fe] or [Ni-Fe-Se] hydrogenase and cytochrome c3 from Desulfovibrio vulgaris Hildenborough or Desulfovibrio desulfuricans Norway have been studied. Some structural implications are considered from these experimental data.
为了理解[Fe]和[Ni-Fe]氢化酶的电子转移机制，已对细胞色素c3还原进行了动力学研究。循环伏安法和控制电位安培法技术已被用于研究细胞色素c3与来自希登伯勒脱硫弧菌的[Fe]氢化酶之间的分子间电子转移反应。已研究了来自希登伯勒脱硫弧菌或挪威脱硫脱硫弧菌的[Fe]或[Ni-Fe-Se]氢化酶与细胞色素c3之间的电子转移交叉反应。从这些实验数据中考虑了一些结构方面的影响。
