Hamakubo T, Furuta H, Ichimura M, Appalsamy M, Mosqueda-Garcia R, Robertson D, Inagami T
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.
Biochem Biophys Res Commun. 1992 Dec 15;189(2):691-6. doi: 10.1016/0006-291x(92)92256-w.
We examined the hypothesis that hypothalamo-hypophysial tissue contains an endogenous Na pump inhibitor. From bovine posterior pituitary, we purified a substance which inhibits Rb uptake by human erythrocytes. This inhibitory activity was found in the eluate of 10% acetonitrile from a C18 flash column and purified by subsequent three steps of reversed-phase high-performance liquid chromatography (HPLC). Sequence analysis revealed that this substance was identical to joining peptide, one of the major products of proopiomelanocortin (POMC). This peptide had hypertensive and tachycardiac effects in spontaneously hypertensive rats (SHR) after central administration, with weak Na,K-ATPase inhibitory activity (IC50 = 0.5 mM).
我们检验了下丘脑 - 垂体组织含有内源性钠泵抑制剂这一假说。从牛垂体后叶中，我们纯化出了一种抑制人红细胞摄取铷的物质。这种抑制活性存在于C18快速柱10%乙腈洗脱液中，并通过后续三步反相高效液相色谱（HPLC）进行纯化。序列分析表明，该物质与阿片促黑素皮质素原（POMC）的主要产物之一连接肽相同。该肽经中枢给药后，对自发性高血压大鼠（SHR）有升压和心动过速作用，且具有较弱的钠钾ATP酶抑制活性（IC50 = 0.5 mM）。
